Analytical Data
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Gene name
SMPD3
- Application
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Alternative Names
SMPD3；Sphingomyelin phosphodiesterase 3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NY59
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Expression Region
86-655aa
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AA Sequence
SPLQSARRPYIYSRLEDKGLAGGAALLSEWKGTGPGKSFCFATANVCLLPDSLARVNNLFNTQARAKEIGQRIRNGAARPQIKIYIDSPTNTSISAASFSSLVSPQGGDGVARAVPGSIKRTASVEYKGDGGRHPGDEAANGPASGDPVDSSSPEDACIVRIGGEEGGRPPEADDPVPGGQARNGAGGGPRGQTPNHNQQDGDSGSLGSPSASRESLVKGRAGPDTSASGEPGANSKLLYKASVVKKAAARRRRHPDEAFDHEVSAFFPANLDFLCLQEVFDKRAATKLKEQLHGYFEYILYDVGVYGCQGCCSFKCLNSGLLFASRYPIMDVAYHCYPNKCNDDALASKGALFLKVQVGSTPQDQRIVGYIACTHLHAPQEDSAIRCGQLDLLQDWLADFRKSTSSSSAANPEELVAFDVVCGDFNFDNCSSDDKLEQQHSLFTHYRDPCRLGPGEEKPWAIGTLLDTNGLYDEDVCTPDNLQKVLESEEGRREYLAFPTSKSSGQKGRKELLKGNGRRIDYMLHAEEGLCPDWKAEVEEFSFITQLSGLTDHLPVAMRLMVSSGEEEA
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Molecular Weight
68.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SMPD3, or sphingomyelin phosphodiesterase 3, is an enzyme that plays a critical role in sphingolipid metabolism, particularly in the hydrolysis of sphingomyelin to generate ceramide and phosphocholine. Sphingolipids are essential components of cellular membranes and are involved in various cellular processes, including apoptosis, cell signaling, and membrane dynamics. Dysregulation of sphingolipid metabolism has been associated with several diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. Research on SMPD3 has gained momentum due to its potential implications in these pathologies. By studying SMPD3 recombinant protein, researchers aim to gain insights into its enzymatic mechanisms, regulatory pathways, and interactions with other molecules. This knowledge can help elucidate its role in disease processes and potentially identify novel therapeutic targets. Furthermore, the production of SMPD3 as a recombinant protein allows for detailed biochemical characterization and the development of assays for drug screening. Understanding the structure and function of SMPD3 at the molecular level may pave the way for innovative treatments that modulate sphingomyelin and ceramide levels, ultimately contributing to advances in the management of diseases linked to sphingolipid metabolism.
