Analytical Data
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Gene name
AP3s2
- Application
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Alternative Names
AP3s2；AP-3 complex subunit sigma-2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P59780
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Expression Region
1-193aa
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AA Sequence
MIQAILVFNN HGKPRLVRFY QRFPEEIQQQ IVRETFHLVL KRDDNICNFL EGGSLIGGSD YKLIYRHYAT LYFVFCVDSS ESELGILDLI QVFVETLDKC FENVCELDLI FHMDKVHYIL QEVVMGGMVL ETNMNEIVAQ IEAQNRLEKS EGGLSAAPAR AVSAVKNINL PEIPRNINIG DLNIKVPNLS QFV
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Molecular Weight
22 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
AP3s2, or Adaptor Protein Complex 3 Sigma-2 subunit, is a crucial component of the AP-3 complex, which plays a significant role in intracellular trafficking, particularly in the transport of proteins to lysosomes and related organelles. Research into AP3s2 has gained attention due to its involvement in various cellular processes, including endocytosis, membrane trafficking, and the sorting of proteins containing specific signals. Deficiencies or mutations in AP3s2 have been linked to several neurodegenerative diseases and developmental disorders, highlighting its biological significance. Understanding the structure and function of AP3s2 at the molecular level can provide insights into its role in these pathological conditions, potentially identifying new therapeutic targets. The recombinant expression of AP3s2 allows for detailed biochemical and biophysical characterization, aiding in the elucidation of its interaction networks and functional mechanisms within the cell. Recent advancements in purification techniques and structural biology, such as cryo-electron microscopy, have further accelerated research efforts aimed at comprehensively mapping the AP3 complex and its diverse roles in cellular homeostasis. By exploring AP3s2 through recombinant protein studies, researchers hope to unravel its complexities and contribute to the broader understanding of vesicular trafficking and its implications in human health and disease.
