Analytical Data
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Gene name
AP2b1
- Application
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Alternative Names
AP2b1；ADTB2；CLAPB1；AP-2 complex subunit beta
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q10567
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Expression Region
1-584aa
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AA Sequence
MTDSKYFTTTKKGEIFELKAELNSDKKEKKKEAVKKVIASMTVGKDVSALFPDVVNCMQTDNLELKKLVYLYLMNYAKSQPDMAIMAVNTFVKDCEDPNPLIRALAVRTMGCIRVDKITEYLCEPLRKCLKDEDPYVRKTAAVCVAKLHDINAQLVEDQGFLDTLKDLISDSNPMVVANAVAALSEIAESHPSSNLLDLNPQSINKLLTALNECTEWGQIFILDCLANYMPKDDREAQSICERVTPRLSHANSAVVLSAVKVLMKFMEMLSKDLDYYGTLLKKLAPPLVTLLSAEPELQYVALRNINLIVQKRPEILKHEMKVFFVKYNDPIYVKLEKLDIMIRLASQANIAQVLAELKEYATEVDVDFVRKAVRAIGRCAIKVEQSAERCVSTLLDLIQTKVNYVVQEAIVVIKDIFRKYPNKYESVIATLCENLDSLDEPEARAAMIWIVGEYAERIDNADELLESFLEGFHDESTQVQLQLLTAIVKLFLKKPTETQELVQQVLSLATQDSDNPDLRDRGYIYWRLLSTDPVAAKEVVLAEKPLISEETDLIEPTLLDELICYIGTLASVYHKPPSAFVEG
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Molecular Weight
73.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AP2b1, a member of the adaptor protein complex family, plays a crucial role in clathrin-mediated endocytosis, which is essential for cellular transport and signaling processes. This protein facilitates the internalization of membrane proteins and regulates various cellular activities by linking membrane proteins to the clathrin coat, enabling the formation of vesicles that transport cargo to different cellular compartments. Research on AP2b1 has gained momentum due to its implications in several physiological and pathological processes; abnormalities in its function can lead to diseases such as cancer and neurological disorders. As a result, the development of recombinant AP2b1 proteins has become a focus of study, aiming to better understand its structure-function relationship and mechanisms of action. By producing AP2b1 in a recombinant form, researchers can investigate its interactions with other proteins, elucidate its role in endocytic trafficking, and explore its potential as a therapeutic target. The study of AP2b1 not only enhances our understanding of cellular dynamics but also opens avenues for novel approaches in drug development and biomarker discovery for diseases linked to endocytic dysregulation. Thus, the investigation of recombinant AP2b1 proteins holds significant promise for advancing both basic science and translational research in cellular biology and medicine.
