Analytical Data
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Gene name
SAR1B
- Application
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Alternative Names
SAR1B;SARA2;SARB;Small COPII coat GTPase SAR1B
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y6B6
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Expression Region
1-198aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSFIFDW IYSGFSSVLQ FLGLYKKTGK LVFLGLDNAG KTTLLHMLKD DRLGQHVPTL HPTSEELTIA GMTFTTFDLG GHVQARRVWK NYLPAINGIV FLVDCADHER LLESKEELDS LMTDETIANV PILILGNKID RPEAISEERL REMFGLYGQT TGKGSISLKE LNARPLEVFM CSVLKRQGYG EGFRWMAQYI D
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Molecular Weight
25 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SAR1B is a key protein involved in the transport of proteins and lipids from the endoplasmic reticulum (ER) to the Golgi apparatus. It is a member of the Sar1 family of small GTPases, which are crucial for the initiation of protein transport via COPII vesicles. Mutations in the SAR1B gene have been linked to various human diseases, particularly a form of congenital disorders of glycosylation known as CDG-IIc. These mutations disrupt the normal functioning of the protein, leading to impaired glycoprotein synthesis and severe clinical manifestations. Research on SAR1B recombinant proteins has gained traction due to its potential implications in understanding the molecular mechanisms underlying these diseases and identifying therapeutic targets. By studying the structure, function, and dynamics of SAR1B, scientists hope to shed light on the role of vesicular transport in health and disease, and develop strategies to mitigate the effects of mutations within this pathway. Additionally, investigating SAR1B activity may provide insights into broader cellular processes, as vesicular transport is fundamental to maintaining cellular homeostasis and intercellular communication. The ongoing research in this field aims to unravel the intricate network of protein trafficking and highlight the significance of SAR1B as a critical component in cellular biology.
