Cat: PA2000-9708

Recombinant Human NIF3L1 Protein,His

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Analytical Data

  • Gene name

    NIF3L1

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    ALS2CR1; Amyotrophic lateral sclerosis 2 (juvenile) chromosome region. candidate 1; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 1 protein; CALS-7; MDS015; Ngg1 interacting factor 3-like 1; NIF3 (Ngg1 interacting factor 3. S.pombe homolog)-like 1; NIF3 NGG1 interacting factor 3-like 1 (S. pombe); NIF3 NGG1 interacting factor 3-like 1; NIF3-like protein 1; NIF3L_HUMAN; Nif3l1; OTTHUMP00000206190 ; OTTHUMP00000206191 ; OTTHUMP00000206192

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q9GZT8

  • Expression Region

    1-377 aa

  • AA Sequence

    MLSSCVRPVP TTVRFVDSLI CNSSRSFMDL KALLSSLNDF ASLSFAESWD NVGLLVEPSP PHTVNTLFLT NDLTEEVMEE VLQKKADLIL SYHPPIFRPM KRITWNTWKE RLVIRALENR VGIYSPHTAY DAAPQGVNNW LAKGLGACTS RPIHPSKAPN YPTEGNHRVE FNVNYTQDLD KVMSAVKGID GVSVTSFSAR TGNEEQTRIN LNCTQKALMQ VVDFLSRNKQ LYQKTEILSL EKPLLLHTGM GRLCTLDESV SLATMIDRIK RHLKLSHIRL ALGVGRTLES QVKVVALCAG SGSSVLQGVE ADLYLTGEMS HHDTLDAASQ GINVILCEHS NTERGFLSDL RDMLDSHLEN KINIILSETD RDPLQVV

  • Molecular Weight

    41.9 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

NIF3L1, a member of the NIF3 (Nuclear Interaction Family), is a protein that has garnered attention due to its potential roles in various cellular processes, including nuclear transport and signal transduction. Recent studies have indicated that NIF3L1 may be involved in the regulation of gene expression and cellular responses to environmental stressors, highlighting its importance in maintaining cellular homeostasis. The recombinant production of NIF3L1 allows researchers to investigate its functional properties and interactions at a molecular level. By expressing this protein in various systems, such as bacterial or mammalian cells, scientists can purify it for biochemical assays, structural studies, and functional analyses, thereby elucidating its specific roles within the cell. Understanding NIF3L1's structure and function could pave the way for new therapeutic strategies, particularly in diseases where nuclear transport and signaling pathways are disrupted. Overall, the study of recombinant NIF3L1 holds significant promise for expanding our knowledge of cellular mechanisms and developing targeted interventions in related disorders.

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