Analytical Data
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基因名
ACSL5
- Application
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别名
ACSL5；ACS5；FACL5；Long-chain-fatty-acid--CoA ligase 5
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种属
Human
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9ULC5
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表达区间
1-683aa
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氨基酸序列
MLFIFNFLFSPLPTPALICILTFGAAIFLWLITRPQPVLPLLDLNNQSVGIEGGARKGVSQKNNDLTSC CFSDAKTMYEVFQRGLAVSDNGPCLGYRKPNQPYRWLSYKQVSDRAEYLGSCLLHKGYKSSPDQ FVGIFAQNRPEWIISELACYTYSMVAVPLYDTLGPEAIVHIVNKADIAMVICDTPQKALVLIGNVEKGF TPSLKVIILMDPFDDDLKQRGEKSGIEILSLYDAENLGKEHFRKPVPPSPEDLSVICFTSGTTGDPKG AMITHQNIVSNAAAFLKCVEHAYEPTPDDVAISYLPLAHMFERIVQAVVYSCGARVGFFQGDIRLLAD DMKTLKPTLFPAVPRLLNRIYDKVQNEAKTPLKKFLLKLAVSSKFKELQKGIIRHDSFWDKLIFAKIQD SLGGRVRVIVTGAAPMSTSVMTFFRAAMGCQVYEAYGQTECTGGCTFTLPGDWTSGHVGVPLAC NYVKLEDVADMNYFTVNNEGEVCIKGTNVFKGYLKDPEKTQEALDSDGWLHTGDIGRWLPNGTLKI IDRKKNIFKLAQGEYIAPEKIENIYNRSQPVLQIFVHGESLRSSLVGVVVPDTDVLPSFAAKLGVKGSF EELCQNQVVREAILEDLQKIGKESGLKTFEQVKAIFLHPEPFSIENGLLTPTLKAKRGELSKYFRTQID SLYEHIQD
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分子量
76kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
ACSL5 (Acyl-CoA Synthetase Long-Chain Family Member 5) is a crucial enzyme that plays a significant role in lipid metabolism by catalyzing the activation of long-chain fatty acids into acyl-CoA derivatives, which are vital for various cellular processes including energy production, membrane formation, and signaling pathways. Dysregulation of ACSL5 has been implicated in several metabolic disorders, including obesity, diabetes, and cancer, highlighting its potential as a therapeutic target. Research into recombinant ACSL5 proteins involves the expression and purification of this enzyme to study its enzymatic activity, substrate specificity, and interaction with other metabolic pathways. Investigations have shown that ACSL5 is not only essential for lipid homeostasis but also influences the inflammation and survival of cancer cells, particularly in certain types of tumors such as breast and colorectal cancers. Understanding the structure-function relationship of ACSL5 through recombinant protein studies can provide insights into its regulatory mechanisms and help identify small molecules or biologics that could modulate its activity. Furthermore, such research can contribute to the development of novel strategies for treating diseases associated with altered lipid metabolism, underscoring the importance of ACSL5 in both basic and applied biomedical research.
