Analytical Data
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Gene name
ACSL5
- Application
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Alternative Names
ACSL5；ACS5；FACL5；Long-chain-fatty-acid--CoA ligase 5
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9ULC5
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Expression Region
1-683aa
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AA Sequence
MLFIFNFLFSPLPTPALICILTFGAAIFLWLITRPQPVLPLLDLNNQSVGIEGGARKGVSQKNNDLTSC CFSDAKTMYEVFQRGLAVSDNGPCLGYRKPNQPYRWLSYKQVSDRAEYLGSCLLHKGYKSSPDQ FVGIFAQNRPEWIISELACYTYSMVAVPLYDTLGPEAIVHIVNKADIAMVICDTPQKALVLIGNVEKGF TPSLKVIILMDPFDDDLKQRGEKSGIEILSLYDAENLGKEHFRKPVPPSPEDLSVICFTSGTTGDPKG AMITHQNIVSNAAAFLKCVEHAYEPTPDDVAISYLPLAHMFERIVQAVVYSCGARVGFFQGDIRLLAD DMKTLKPTLFPAVPRLLNRIYDKVQNEAKTPLKKFLLKLAVSSKFKELQKGIIRHDSFWDKLIFAKIQD SLGGRVRVIVTGAAPMSTSVMTFFRAAMGCQVYEAYGQTECTGGCTFTLPGDWTSGHVGVPLAC NYVKLEDVADMNYFTVNNEGEVCIKGTNVFKGYLKDPEKTQEALDSDGWLHTGDIGRWLPNGTLKI IDRKKNIFKLAQGEYIAPEKIENIYNRSQPVLQIFVHGESLRSSLVGVVVPDTDVLPSFAAKLGVKGSF EELCQNQVVREAILEDLQKIGKESGLKTFEQVKAIFLHPEPFSIENGLLTPTLKAKRGELSKYFRTQID SLYEHIQD
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Molecular Weight
76kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
ACSL5 (Acyl-CoA Synthetase Long-Chain Family Member 5) is a crucial enzyme that plays a significant role in lipid metabolism by catalyzing the activation of long-chain fatty acids into acyl-CoA derivatives, which are vital for various cellular processes including energy production, membrane formation, and signaling pathways. Dysregulation of ACSL5 has been implicated in several metabolic disorders, including obesity, diabetes, and cancer, highlighting its potential as a therapeutic target. Research into recombinant ACSL5 proteins involves the expression and purification of this enzyme to study its enzymatic activity, substrate specificity, and interaction with other metabolic pathways. Investigations have shown that ACSL5 is not only essential for lipid homeostasis but also influences the inflammation and survival of cancer cells, particularly in certain types of tumors such as breast and colorectal cancers. Understanding the structure-function relationship of ACSL5 through recombinant protein studies can provide insights into its regulatory mechanisms and help identify small molecules or biologics that could modulate its activity. Furthermore, such research can contribute to the development of novel strategies for treating diseases associated with altered lipid metabolism, underscoring the importance of ACSL5 in both basic and applied biomedical research.
