Analytical Data
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基因名
RPN2
- Application
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别名
RPN2;Dolichyl-diphosphooligosaccharide--Protein glycosyltransferase subunit 2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P04844
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表达区间
23-540aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MLTPTHYLTK HDVERLKASL DRPFTNLESA FYSIVGLSSL GAQVPDAKKA CTYIRSNLDP SNVDSLFYAA QASQALSGCE ISISNETKDL LLAAVSEDSS VTQIYHAVAA LSGFGLPLAS QEALSALTAR LSKEETVLAT VQALQTASHL SQQADLRSIV EEIEDLVARL DELGGVYLQF EEGLETTALF VAATYKLMDH VGTEPSIKED QVIQLMNAIF SKKNFESLSE AFSVASAAAV LSHNRYHVPV VVVPEGSASD THEQAILRLQ VTNVLSQPLT QATVKLEHAK SVASRATVLQ KTSFTPVGDV FELNFMNVKF SSGYYDFLVE VEGDNRYIAN TVELRVKIST EVGITNVDLS TVDKDQSIAP KTTRVTYPAK AKGTFIADSH QNFALFFQLV DVNTGAELTP HQTFVRLHNQ KTGQEVVFVA EPDNKNVYKF ELDTSERKIE FDSASGTYTL YLIIGDATLK NPILWNVADV VIKFPEEEAP STVLSQNLFT PKQEIQHLFR EPEKRPPTV
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分子量
59 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RPN2, a vital component of the 26S proteasome, plays a crucial role in the ubiquitin-proteasome pathway, which is essential for protein degradation and turnover in eukaryotic cells. This protein functions as a regulatory subunit and is implicated in various cellular processes, including cell cycle regulation, signal transduction, and maintaining protein homeostasis. Dysregulation of RPN2 has been associated with several diseases, notably cancers and neurodegenerative disorders, highlighting its significance in maintaining cellular health. Research on recombinant RPN2 protein has gained traction as scientists aim to elucidate its structural and functional characteristics, contributing to a better understanding of proteasome dynamics. By producing and characterizing RPN2 in a recombinant form, researchers can explore its interactions with other proteasome components and regulatory proteins, paving the way for potential therapeutic interventions. The study of RPN2 not only enhances our comprehension of proteolytic mechanisms but also holds promise for the development of novel strategies to combat diseases linked to proteasome dysfunction.
