Analytical Data
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Gene name
SLC39A4
- Application
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Alternative Names
SLC39A4；ZIP4；Zinc transporter ZIP4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6P5W5
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Expression Region
1-647aa
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AA Sequence
MASLVSLELGLLLAVLVVTATASPPAGLLSLLTSGQGALDQEALGGLLNTLADRVHCANGPCGKCLSVEDALGLGEPEGSGLPPGPVLEARYVARLSAAAVLYLSNPEGTCEDARAGLWASHADHLLALLESPKALTPGLSWLLQRMQARAAGQTPKMACVDIPQLLEEAVGAGAPGSAGGVLAALLDHVRSGSCFHALPSPQYFVDFVFQQHSSEVPMTLAELSALMQRLGVGREAHSDHSHRHRGASSRDPVPLISSSNSSSVWDTVCLSARDVMAAYGLSEQAGVTPEAWAQLSPALLQQQLSGACTSQSRPPVQDQLSQSERYLYGSLATLLICLCAVFGLLLLTCTGCRGVTHYILQTFLSLAVGAVTGDAVLHLTPKVLGLHTHSEEGLSPQPTWRLLAMLAGLYAFFLFENLFNLLLPRDPEDLEDGPCGHSSHSHGGHSHGVSLQLAPSELRQPKPPHEGSRADLVAEESPELLNPEPRRLSPELRLLPYMITLGDAVHNFADGLAVGAAFASSWKTGLATSLAVFCHELPHELGDFAALLHAGLSVRQALLLNLASALTAFAGLYVALAVGVSEESEAWILAVATGLFLYVALCDMLPAMLKVRDPRPWLLFLLHNVGLLGGWTVLLLLSLYEDDITF
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Molecular Weight
68.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SLC39A4, also known as ZIP4, is a member of the solute carrier family that plays a crucial role in zinc homeostasis and transport across cellular membranes. The importance of zinc as an essential trace element is well documented, as it is fundamental for numerous biological processes including enzymatic reactions, gene expression, and immune function. Mutations in the SLC39A4 gene have been linked to zinc deficiency syndromes, notably Acrodermatitis Enteropathica, which is characterized by severe skin lesions, diarrhea, and growth retardation. Research on the recombinant protein of SLC39A4 is significant for several reasons. First, it provides insights into the molecular mechanisms underpinning zinc absorption and distribution in cells, contributing to our understanding of related disorders. Second, performing expression and purification of recombinant SLC39A4 allows for functional studies that can reveal the protein's interaction with various ligands and cellular components. Third, structural studies of the recombinant SLC39A4 protein can elucidate its transport mechanism, potentially informing therapeutic strategies for zinc deficiency and related diseases. Overall, the investigation of SLC39A4’s recombinant protein opens up avenues for both basic research and clinical applications, highlighting the interplay between nutrition, metabolism, and genetic factors in maintaining cellular and systemic zinc levels.
