Analytical Data
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Gene name
NDUFS8
- Application
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Alternative Names
CI 23kD; CI-23kD; Complex I 23kD ; Complex I-23kD; EC 1.6.5.3 ; EC 1.6.99.3; Human mitochondrial NADH dehydrogenase ubiquinone Fe S protein 8; NADH dehydrogenase [ubiquinone] iron-sulfur protein 8; mitochondrial; NADH ubiquinone oxidoreductase 23 kDa subunit ; NADH-ubiquinone oxidoreductase 23 kDa subunit; NDUFS8; NDUS8_HUMAN; TYKY ; TYKY subunit; Ubiquinoneiron sulfur protein 8; mitochondrial precursor
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O00217
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Expression Region
35-210 aa
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AA Sequence
TYKYVN MQDPEMDMKS VTDRAARTLL WTELFRGLGM TLSYLFREPA TINYPFEKGP LSPRFRGEHA LRRYPSGEER CIACKLCEAI CPAQAITIEA EPRADGSRRT TRYDIDMTKC IYCGFCQEAC PVDAIVEGPN FEFSTETHEE LLYNKEKLLN NGDKWEAEIA ANIQADYLYR
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Molecular Weight
23.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NDUFS8 is a key component of the mitochondrial complex I, also known as NADH:ubiquinone oxidoreductase, which plays a crucial role in the oxidative phosphorylation pathway, essential for ATP production in eukaryotic cells. Research into NDUFS8 has gained significance due to its implications in mitochondrial diseases and neurodegenerative disorders. Mutations in the NDUFS8 gene can lead to impaired complex I activity, resulting in reduced ATP synthesis and increased production of reactive oxygen species, both of which are associated with various pathologies, including Leigh syndrome, a severe neurological disorder. Understanding the structure and function of recombinant NDUFS8 protein has become a focus of biochemical and molecular studies, as it may provide insights into its mechanistic role within the electron transport chain. Furthermore, characterizing this protein can facilitate the development of therapeutic strategies aimed at mitigating the effects of mitochondrial dysfunction. Advances in protein engineering and recombinant DNA technology allow for the production of NDUFS8 in heterologous systems, enabling detailed functional assays and structural studies that can elucidate the dynamics of complex I assembly and activity. Consequently, NDUFS8 serves as a vital target for research into mitochondrial health, the pathogenesis of related diseases, and the exploration of potential treatments to restore or improve mitochondrial function.
