Analytical Data
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Gene name
NDUFS7
- Application
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Alternative Names
CI 20; CI-20kD; Complex I 20kDa subunit; Complex I mitochondrial respiratory chain 20 KD subunit; Complex I-20kD; FLJ45860; FLJ46880; MGC120002; MY017; NADH coenzyme Q reductase; NADH dehydrogenase (ubiquinone) Fe S protein 7 20kDa (NADH coenzyme Q reductase); NADH dehydrogenase (ubiquinone) FeS protein 7; 20kDa (NADHcoenzyme Q reductase) ; NADH dehydrogenase (ubiquinone) FeS protein7; 20kDa (NADHcoenzyme Q reductase); NADH dehydrogenase [ubiquinone] iron-sulfur protein 7; mitochondrial; NADH-ubiquinone oxidoreductase 20 kDa subunit; NADH:ubiquinone oxidoreductase PSST subunit ; NADHcoenzyme Q reductase; Ndufs7; NDUS7_HUMAN; PSST; PSST subunit
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O75251
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Expression Region
39-213 aa
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AA Sequence
PS STQPALPKAR AVAPKPSSRG EYVVAKLDDL VNWARRSSLW PMTFGLACCA VEMMHMAAPR YDMDRFGVVF RASPRQSDVM IVAGTLTNKM APALRKVYDQ MPEPRYVVSM GSCANGGGYY HYSYSVVRGC DRIVPVDIYI PGCPPTAEAL LYGILQLQRK IKRERRLQIW YRR
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Molecular Weight
23.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NDUFS7, a vital component of the mitochondrial respiratory chain complex I, plays a crucial role in cellular energy production through oxidative phosphorylation. Research into NDUFS7 has gained traction due to its implication in various mitochondrial disorders, particularly those linked to complex I deficiencies. Mutations in the NDUFS7 gene can lead to severe metabolic disturbances, presenting in conditions such as Leigh syndrome and other neurodegenerative disorders characterized by impaired energy metabolism. Understanding the structure and function of the NDUFS7 protein is essential for elucidating the mechanisms of these diseases and developing potential therapeutic strategies. Recombinant NDUFS7 proteins are increasingly being utilized in biochemical studies to explore their interactions with other subunits of complex I and elucidate their role in mitochondrial function. By producing and studying recombinant forms of NDUFS7, researchers aim to investigate the protein’s stability, folding properties, and interactions within the mitochondrial matrix, which could lead to insights into the pathophysiology of associated diseases and advancements in gene therapy or enzyme replacement strategies. Overall, the study of recombinant NDUFS7 is significant for both basic mitochondrial biology and the clinical understanding of complex I-related disorders.
