Analytical Data
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基因名
NDUFB6
- Application
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别名
NDUFB6; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6; Complex I-B17; CI-B17; NADH-ubiquinone oxidoreductase B17 subunit
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O95139
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表达区间
1-128 aa
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氨基酸序列
MTGYTPDEKLRLQQLRELRRRWLKDQELSPREPVLPPQKMGPMEKFWNKFLENKSPWRKMVHGVYKKSIFVFTHVLVPVWIIHYYMKYHVSEKPYGIVEKKSRIFPGDTILETGEVIPPMKEFPDQHH
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分子量
39.82 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NDUFB6, a subunit of the mitochondrial NADH-ubiquinone oxidoreductase (Complex I), plays a critical role in the mitochondrial electron transport chain, facilitating the conversion of NADH into ubiquinone while contributing to the generation of a proton gradient necessary for ATP synthesis. Mutations and dysfunctions in NDUFB6 have been implicated in various mitochondrial disorders, leading to a spectrum of pathologies, including neurodegenerative diseases and myopathies. The study of recombinant NDUFB6 protein is important for understanding its structural and functional properties, as well as its interactions within the larger Complex I assembly. Researchers aim to elucidate the mechanistic details of its role in electron transfer and its potential involvement in the regulation of oxidative phosphorylation. By producing recombinant NDUFB6, scientists can perform biochemical assays, investigate its interactions with other complex I subunits, and assess the impact of specific mutations. This research not only contributes to our understanding of mitochondrial biology but also has implications for the development of therapeutic strategies targeting mitochondrial dysfunction. Thus, the study of NDUFB6 at a molecular level is paramount for unraveling the complexities of cellular energy metabolism and addressing mitochondrial-related diseases.
