Analytical Data
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Gene name
RNF114
- Application
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Alternative Names
RNF114;ZNF228;ZNF313;E3 ubiquitin-Protein ligase RNF114
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y508
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Expression Region
1-228aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAAQQRD CGGAAQLAGP AAEADPLGRF TCPVCLEVYE KPVQVPCGHV FCSACLQECL KPKKPVCGVC RSALAPGVRA VELERQIEST ETSCHGCRKN FFLSKIRSHV ATCSKYQNYI MEGVKATIKD ASLQPRNVPN RYTFPCPYCP EKNFDQEGLV EHCKLFHSTD TKSVVCPICA SMPWGDPNYR SANFREHIQR RHRFSYDTFV DYDVDEEDMM NQVLQRSIID Q
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Molecular Weight
28 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RNF114, a member of the RBR E3 ubiquitin ligase family, has garnered significant attention in recent years due to its pivotal role in cellular processes such as protein degradation, signal transduction, and immune responses. Research has revealed that RNF114 is involved in the ubiquitination of specific target proteins, which is crucial for maintaining cellular homeostasis and regulating various biological functions. Its expression is highly regulated and often linked to various diseases, including cancer and autoimmune disorders. Scientists are particularly interested in understanding the molecular mechanisms by which RNF114 exerts its functions and how its dysregulation can lead to pathological conditions. Additionally, the development of recombinant RNF114 proteins allows for an in-depth exploration of its enzymatic activity and substrate specificity, facilitating the identification of potential therapeutic targets. By elucidating the structure-function relationship of RNF114, researchers aim to uncover novel insights that could inform strategies for intervention in diseases associated with its dysfunction. This growing body of research emphasizes the importance of RNF114 in both basic biological research and its potential implications in the field of medicine, thus highlighting the need for continued study of this intriguing protein.
