Analytical Data
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基因名
NDUFB3
- Application
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别名
NDUFB3; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3; Complex I-B12; CI-B12; NADH-ubiquinone oxidoreductase B12 subunit
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O43676
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表达区间
1-98 aa
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氨基酸序列
MAHEHGHEHGHHKMELPDYRQWKIEGTPLETIQKKLAAKGLRDPWGRNEAWRYMGGFAKSVSFSDVFFKGFKWGFAAFVVAVGAEYYLESLNKDKKHH
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分子量
36.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NDUFB3, a crucial component of the mitochondrial respiratory chain, is a subunit of complex I (NADH:ubiquinone oxidoreductase), which plays a pivotal role in cellular energy production through oxidative phosphorylation. Mutations in the NDUFB3 gene are associated with various mitochondrial disorders, leading to impaired energy metabolism and a range of clinical manifestations. Given the enzyme's essential function in ATP synthesis and its involvement in mitochondrial pathophysiology, research on NDUFB3 recombinant protein has garnered significant attention. Studies have focused on the characterization of its structure and function, understanding the molecular mechanisms underlying its role in electron transport, and investigating the effects of specific mutations on its activity. Additionally, recombinant NDUFB3 protein is increasingly utilized in the development of therapeutic strategies aimed at correcting mitochondrial dysfunction. By exploring the biochemical properties and interactions of NDUFB3 within the respiratory complex, researchers aim to uncover potential targets for intervention in mitochondrial diseases, thereby paving the way for novel treatments and enhancing our understanding of mitochondrial biology.
