Analytical Data
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Gene name
HMGN5
- Application
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Alternative Names
HMGN5；NSBP1；High mobility group nucleosome-binding domain-containing Protein 5
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P82970
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Expression Region
1-282aa
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AA Sequence
MPKRKAAGQG DMRQEPKRRS ARLSAMLVPV TPEVKPKRTS SSRKMKTKSD MMEENIDTSA QAVAETKQEA VVEEDYNENA KNGEAKITEA PASEKEIVEV KEENIEDATE KGGEKKEAVA AEVKNEEEDQ KEDEEDQNEE KGEAGKEDKD EKGEEDGKED KNGNEKGEDA KEKEDGKKGE DGKGNGEDGK EKGEDEKEEE DRKETGDGKE NEDGKEKGDK KEGKDVKVKE DEKEREDGKE DEGGNEEEAG KEKEDLKEEE EGKEEDEIKE DDGKKEEPQS IV
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Molecular Weight
31.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HMGN5, a member of the high-mobility group nucleosomal protein family, plays a crucial role in chromatin remodeling and gene expression regulation. Recent studies have highlighted its significance in various cellular processes, including differentiation, proliferation, and response to stress. The protein is known to facilitate the binding of transcription factors to nucleosomal DNA, thus modulating the accessibility of genetic material for transcription. Increased expression of HMGN5 has been linked to numerous types of cancer, where it may contribute to malignant transformation and tumor progression. As a result, HMGN5 has drawn considerable attention for its potential as a therapeutic target and biomarker in oncology. The generation of recombinant HMGN5 protein is essential for further studies aimed at understanding its structure-function relationship, interaction with other cellular components, and its role in pathological conditions. Various expression systems, including bacterial and eukaryotic cell lines, have been explored for the efficient production of HMGN5, which can facilitate the investigation of its biological functions through biochemical assays, structural analyses, and functional studies. Moreover, understanding how HMGN5 interacts with chromatin and other nuclear proteins may unveil novel insights into epigenetic regulation and cancer biology, emphasizing the importance of continued research in this area.
