Analytical Data
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基因名
NDUFA3
- Application
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别名
NDUFA3; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3; Complex I-B9; CI-B9; NADH-ubiquinone oxidoreductase B9 subunit
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O95167
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表达区间
2-84 aa
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氨基酸序列
AARVGAFLKNAWDKEPVLVVSFVVGGLAVILPPLSPYFKYSVMINKATPYNYPVPVRDDGNMPDVPSHPQDPQGPSLEWLKKL
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分子量
36.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NDUFA3 (NADH:ubiquinone oxidoreductase subunit A3) is a vital component of the mitochondrial complex I, which plays a crucial role in the cellular energy metabolism by facilitating the transfer of electrons from NADH to ubiquinone. Mutations or deficiencies in NDUFA3 have been linked to diverse mitochondrial disorders, resulting in a range of clinical manifestations, including neurological deficits and myopathies. Given its essential role in mitochondrial function, the study of NDUFA3 and its recombinant protein is significant for understanding the pathogenesis of related diseases and exploring potential therapeutic strategies. Recent advances in protein engineering techniques have enabled the successful expression and purification of recombinant NDUFA3, allowing for in-depth functional assays and structural studies. These investigations not only enhance our comprehension of the mechanistic roles of NDUFA3 in the electron transport chain but also provide insights into the regulation of mitochondrial bioenergetics. Furthermore, understanding the biochemical properties and interactions of recombinant NDUFA3 can pave the way for the development of targeted therapies aimed at correcting the defective mitochondrial functions associated with NDUFA3 mutations. Overall, the research on NDUFA3 recombinant protein is pivotal for unraveling the complexities of mitochondrial biology and addressing the challenges posed by mitochondrial diseases.
