Analytical Data
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Gene name
NCOA5
- Application
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Alternative Names
NCOA5; KIAA1637; Nuclear receptor coactivator 5; NCoA-5; Coactivator independent of AF-2; CIA
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9HCD5
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Expression Region
1-579 aa
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AA Sequence
MNTAPSRPSP TRRDPYGFGD SRDSRRDRSP IRGSPRREPR DGRNGRDARD SRDIRDPRDL RDHRHSRDLR DHRDSRSVRD VRDVRDLRDF RDLRDSRDFR DQRDPMYDRY RDMRDSRDPM YRREGSYDRY LRMDDYCRRK DDSYFDRYRD SFDGRGPPGP ESQSRAKERL KREERRREEL YRQYFEEIQR RFDAERPVDC SVIVVNKQTK DYAESVGRKV RDLGMVVDLI FLNTEVSLSQ ALEDVSRGGS PFAIVITQQH QIHRSCTVNI MFGTPQEHRN MPQADAMVLV ARNYERYKNE CREKEREEIA RQAAKMADEA ILQERERGGP EEGVRGGHPP AIQSLINLLA DNRYLTAEET DKIINYLRER KERLMRSSTD SLPGPISRQP LGATSGASLK TQPSSQPLQS GQVLPSATPT PSAPPTSQQE LQAKILSLFN SGTVTANSSS ASPSVAAGNT PNQNFSTAAN SQPQQRSQAS GNQPPSILGQ GGSAQNMGPR PGAPSQGLFG QPSSRLAPAS NMTSQRPVSS TGINFDNPSV QKALDTLIQS GPALSHLVSQ TTAQMGQPQA PMGSYQRHY
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Molecular Weight
65.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NCOA5, or Nuclear Receptor Coactivator 5, is a protein that plays a crucial role in the regulation of gene expression by acting as a coactivator for various nuclear hormone receptors. It is involved in several physiological processes, including metabolism, reproduction, and development. Recent studies have implicated NCOA5 in various pathologies, including obesity, diabetes, and certain types of cancer, highlighting its potential as a therapeutic target. The significance of NCOA5 in cellular signaling pathways and its interactions with other proteins make it a key player in the regulation of downstream gene activation. However, research on NCOA5 has been limited by the difficulty in obtaining it in a pure form, which is essential for functional studies. The production of recombinant NCOA5 protein offers a promising approach to elucidate its structure, function, and molecular interactions in vivo. By employing techniques such as recombinant DNA technology and expression in suitable host systems, researchers aim to generate sufficient quantities of the protein for biochemical analysis and crystallization. This progress will enhance the understanding of NCOA5's role in health and disease and could pave the way for novel interventions in related disorders. The investigation of NCOA5 in various disease contexts continues to reveal its complex biological functions, cementing its importance in the field of molecular biology and medicine.
