Analytical Data
-
Gene name
REV1
- Application
-
Alternative Names
REV1;REV1L;DNA repair Protein REV1
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q9UBZ9-1
-
Expression Region
51-256aa
-
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGVAIYVNGY TDPSAEELRK LMMLHGGQYH VYYSRSKTTH IIATNLPNAK IKELKGEKVI RPEWIVESIK AGRLLSYIPY QLYTKQSSVQ KGLSFNPVCR PEDPLPGPSN IAKQLNNRVN HIVKKIETEN EVKVNGMNSW NEEDENNDFS FVDLEQTSPG RKQNGIPHPR GSTAIFNGHT PSSNGALKTQ DCLVPMVNSV ASRLSPA
-
Molecular Weight
25 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
REV1 is a specialized DNA polymerase belonging to the Y-family of polymerases, which plays a crucial role in the cellular response to DNA damage, particularly during the process of translesion synthesis (TLS). TLS is a mechanism that allows cells to bypass DNA lesions that would otherwise stall replication forks, thereby preventing potential cell death or mutations. In various organisms, REV1 has been implicated in mitigating the effects of genotoxic stress by incorporating nucleotides opposite damaged bases, facilitating DNA repair, and maintaining genomic stability. Research on REV1 has gained momentum due to its dual role in both promoting cellular survival in response to DNA damage and its involvement in mutagenesis, which can contribute to cancer progression. Understanding the structural and functional properties of REV1, as well as its interactions with other proteins involved in the TLS pathway, provides insights into how cells cope with DNA insults. Moreover, exploring REV1's activity could have therapeutic implications for cancer treatment strategies, particularly in drugs that target replicative DNA polymerases or exploit the DNA damage response mechanisms. Enhanced knowledge of REV1's function and regulation in different cellular contexts is essential for developing targeted therapies that may enhance the efficacy of existing treatments or target specific cancer cells more effectively.
