Analytical Data
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Gene name
RBM17
- Application
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Alternative Names
RBM17;SPF45;Splicing factor 45
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96I25
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Expression Region
1-401aa
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AA Sequence
MGSSHHHHHHSSGLVPRGSHMSLYDDLGVETSDSKTEGWSKNFKLLQSQL QVKKAALTQAKSQRTKQSTVLAPVIDLKRGGSSDDRQIVDTPPHVAAGLK DPVPSGFSAGEVLIPLADEYDPMFPNDYEKVVKRQREERQRQRELERQKE IEEREKRRKDRHEASGFARRPDPDSDEDEDYERERRKRSMGGAAIAPPTS LVEKDKELPRDFPYEEDSRPRSQSSKAAIPPPVYEEQDRPRSPTGPSNSF LANMGGTVAHKIMQKYGFREGQGLGKHEQGLSTALSVEKTSKRGGKIIVG DATEKDASKKSDSNPLTEILKCPTKVVLLRNMVGAGEVDEDLEVETKEEC EKYGKVGKCVIFEIPGAPDDEAVRIFLEFERVESAIKAVVDLNGRYFGGR VVKACFYNLDKFRVLDLAEQV
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Molecular Weight
47 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RBM17 (RNA-binding motif protein 17) is an essential splicing factor that plays a critical role in the regulation of gene expression and alternative splicing processes. Research has shown that RBM17 is involved in various cellular functions, including cell proliferation, differentiation, and apoptosis, making it significant in both normal biological processes and pathological conditions such as cancer. The protein interacts with pre-mRNA transcripts, influencing the splicing machinery's decision-making, which can result in the production of multiple protein isoforms from the same gene. Dysregulation of RBM17 has been associated with several diseases, particularly in cancer, where it can affect the expression of oncogenes and tumor suppressor genes. Given its importance in RNA processing and its potential role as a therapeutic target, researchers have focused on the structural and functional characterization of RBM17 through recombinant protein studies. These studies involve the production of RBM17 in various expression systems, allowing for the investigation of its biochemical properties, interaction partners, and functional implications in cell biology. Understanding the mechanisms by which RBM17 regulates splicing and its interactions with other cellular components is crucial for deciphering its role in health and disease, paving the way for potential therapeutic interventions targeting splicing pathways influenced by RBM17.
