Analytical Data
-
Gene name
NANOS3
- Application
-
Alternative Names
NANO3_HUMAN; Nanos homolog 3; NANOS3; NOS-3; Nos3
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P60323
-
Expression Region
1-173 aa
-
AA Sequence
MGTFDLWTDY LGLAHLVRAL SGKEGPETRL SPQPEPEPML EPDQKRSLES SPAPERLCSF CKHNGESRAI YQSHVLKDEA GRVLCPILRD YVCPQCGATR ERAHTRRFCP LTGQGYTSVY SHTTRNSAGK KLVRPDKAKT QDTGHRRGGG GGAGFRGAGK SEPSPSCSPS MST
-
Molecular Weight
18.8 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NANOS3 is a member of the NANOS protein family, crucial for germ cell development and maintenance in various organisms, including mammals. Research into NANOS3 has gained significance due to its pivotal role in the regulation of germ cell fate, particularly in the context of spermatogenesis and oogenesis. These proteins are involved in post-transcriptional regulation, influencing mRNA stability and translation, which are essential for proper germ cell function and differentiation. Abnormalities in NANOS3 expression or function have been linked to reproductive disorders and infertility. Studies have shown that NANOS3 interacts with specific RNA-binding proteins and microRNAs, which may offer insights into the molecular mechanisms governing germ cell development. The investigation of NANOS3 not only elucidates fundamental biological processes but also has potential implications for reproductive medicine and the development of therapeutic strategies for infertility. Furthermore, given the evolutionary conservation of NANOS proteins, research on NANOS3 can provide comparative insights into germ cell biology across species, making it a critical focal point in developmental biology and reproductive health research.
