Cat: PA1000-2672

Recombinant Human RASSF1A Protein,His

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Analytical Data

  • Gene name

    RASSF1A

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    RASSF1;RDA32;Ras association domain-containing Protein 1

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q9NS23

  • Expression Region

    2-344aa

  • AA Sequence

    SGEPELIEL RELAPAGRAG KGRTRLERAN ALRIARGTAC NPTRQLVPGR GHRFQPAGPA THTWCDLCGD FIWGVVRKGL QCARLSADCK FTCHYRCRAL VCLDCCGPRD LGWEPAVERD TNVDEPVEWE TPDLSQAEIE QKIKEYNAQI NSNLFMSLNK DGSYTGFIKV QLKLVRPVSV PSSKKPPSLQ DARRGPGRGT SVRRRTSFYL PKDAVKHLHV LSRTRAREVI EALLRKFLVV DDPRKFALFE RAERHGQVYL RKLLDDEQPL RLRLLAGPSD KALSFVLKEN DSGEVNWDAF SMPELHNFLR ILQREEEEHL RQILQKYSYC RQKIQEALHA CPLG

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

RASSF1A (Ras Association Domain Family 1 Isoform A) is a crucial tumor suppressor protein that plays a significant role in various cellular processes, including cell cycle regulation, apoptosis, and microtubule stabilization. Research has shown that RASSF1A is frequently epigenetically silenced or lost in various cancers, implicating its status as a potential biomarker and therapeutic target. The protein is involved in signaling pathways that regulate cell growth and death, particularly in response to Ras and other oncogenic signals. Its ability to interact with other key proteins like MST1/2 kinases in the Hippo signaling pathway highlights its importance in maintaining normal cellular function and preventing uncontrolled proliferation. Recent studies have focused on understanding the mechanisms regulating RASSF1A expression, exploring its potential as a prognostic indicator, and investigating the therapeutic implications of restoring its function in cancer treatment. This research underscores the pressing need to further elucidate the biology of RASSF1A, how its dysregulation contributes to tumorigenesis, and its viability as a target for novel cancer therapies.

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