Analytical Data
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Gene name
NAF1
- Application
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Alternative Names
NAF1; H/ACA ribonucleoprotein complex non-core subunit NAF1; hNAF1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96HR8
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Expression Region
1-494 aa
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AA Sequence
MEVVEAAAAQ LETLKFNGTD FGVGEGPAAP SPGSAPVPGT QPPLQSFEGS PDAGQTVEVK PAGEQPLQPV LNAVAAGTPA PQPQPPAESP ACGDCVTSPG AAEPARAPDS LETSDSDSDS DSETDSDSSS SSSSSSSSSS SSSSSCISLP PVLSDGDDDL QIEKENKNFP LKTKDELLLN ELPSVEELTI ILPEDIELKP LGMVSSIIEQ LVIIESMTNL PPVNEETVIF KSDRQAAGKI FEIFGPVAHP FYVLRFNSSD HIESKGIKIK ETMYFAPSMK DFTQYIFTEK LKQDKGSDAS WKNDQEPPPE ALDFSDDEKE KEAKQRKKSQ IQGRKKLKSE FNEPGEDFTE VHQNWNAHSS ASEHAKGYRN REFTRGFSRA RYPRSCHGRP PPQHFYNSEH MVSQETSGFP SQRQNNPIMP QYPFPLPVFD MHNFPLRPPP PPPPPPVNMG WATPNMAAHP LLNLPYSLPP PPPPPPLPPP PSSGDSNSHF GPYY
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Molecular Weight
53.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NAF1 (Nuclear Assembly Factor 1) is an essential protein involved in ribosome biogenesis, specifically playing a critical role in the maturation of pre-rRNA and the assembly of ribonucleoprotein complexes. Its importance is underscored by its involvement in various cellular processes, including cell proliferation and response to stress, making it a focal point of study in cellular and molecular biology. Research has highlighted NAF1's interactions with other ribosomal components and its potential implications in diseases such as cancer, where dysregulation of ribosome biogenesis is often observed. Recent advances in recombinant protein technology have facilitated the production and purification of NAF1, allowing for in-depth functional analyses and structural studies. Furthermore, understanding the mechanistic roles of NAF1 in ribosome assembly can provide insights into the fundamental processes of gene expression and cellular growth, as well as contribute to the development of targeted therapeutic strategies for diseases linked to ribosome dysfunction. This growing body of research emphasizes NAF1 as a crucial player not only in ribosome biogenesis but also as a potential biomarker for diseases associated with aberrant protein synthesis, thereby broadening the scope of its significance in both basic and applied biological research.
