Analytical Data
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Gene name
7-DHC
- Application
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Alternative Names
7-DHC；D7SR；7-dehydrocholesterol reductase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UBM7
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Expression Region
1-475aa
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AA Sequence
MAAKSQPNIPKAKSLDGVTNDRTASQGQWGRAWEVDWFSLASVIFLLLFAPFIVYYFIMACDQYSCALTGPVVDIVTGHARLSDIWAKTPPITRKAAQLYTLWVTFQVLLYTSLPDFCHKFLPGYVGGIQEGAVTPAGVVNKYQINGLQAWLLTHLLWFANAHLLSWFSPTIIFDNWIPLLWCANILGYAVSTFAMVKGYFFPTSARDCKFTGNFFYNYMMGIEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAAKQRELHSHVTNAMVLVNVLQAIYVIDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQLSTPHAVGVLLLGLVGYYIFRVANHQKDLFRRTDGRCLIWGRKPKVIECSYTSADGQRHHSKLLVSGFWGVARHFNYVGDLMGSLAYCLACGGGHLLPYFYIIYMAILLTHRCLRDEHRCASKYGRDWERYTAAVPYRLLPGIF
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Molecular Weight
54.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The 7-DHC (7-dehydrocholesterol) protein has gained research interest due to its pivotal role in cholesterol metabolism and its implications in various health conditions. 7-DHC serves as an intermediate in the biosynthesis of cholesterol and is a precursor to vitamin D3, which is vital for bone health and immune function. Abnormal levels of 7-DHC have been associated with several disorders, including Smith-Lemli-Opitz syndrome, a genetic condition resulting in developmental and functional abnormalities. The study of 7-DHC is also significant in the context of cardiovascular diseases, as cholesterol metabolism directly influences lipid profiles and atherosclerosis development. Moreover, recent studies have indicated that alterations in 7-DHC levels may be linked to neurodegenerative diseases, highlighting its potential role in brain health. Advancements in recombinant protein technology allow for the production of 7-DHC in controlled settings, facilitating detailed investigations into its biochemical mechanisms and therapeutic applications. Understanding the intricacies of 7-DHC's function and regulation is crucial for developing innovative approaches to manage related health issues, making it a focal point in current biomedical research initiatives.
