Analytical Data
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Gene name
RFC2
- Application
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Alternative Names
RFC2；Replication factor C subunit 2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P35250
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Expression Region
1-354aa
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AA Sequence
MEVEAVCGGA GEVEAQDSDP APAFSKAPGS AGHYELPWVE KYRPVKLNEI VGNEDTVSRL EVFAREGNVP NIIIAGPPGT GKTTSILCLA RALLGPALKD AMLELNASND RGIDVVRNKI KMFAQQKVTL PKGRHKIIIL DEADSMTDGA QQALRRTMEI YSKTTRFALA CNASDKIIEP IQSRCAVLRY TKLTDAQILT RLMNVIEKER VPYTDDGLEA IIFTAQGDMR QALNNLQSTF SGFGFINSEN VFKVCDEPHP LLVKEMIQHC VNANIDEAYK ILAHLWHLGY SPEDIIGNIF RVCKTFQMAE YLKLEFIKEI GYTHMKIAEG VNSLLQMAGL LARLCQKTMA PVAS
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Molecular Weight
39.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RFC2, or Replication Factor C subunit 2, is a crucial component of the proliferating cell nuclear antigen (PCNA) clamp loader complex, which plays a significant role in DNA replication, repair, and cell cycle regulation. The study of RFC2 has garnered attention due to its involvement in various cellular processes and potential implications in cancer biology, as aberrant DNA replication and repair mechanisms are hallmarks of tumorigenesis. Research indicates that RFC2 is essential for the proper loading of PCNA onto DNA, facilitating the recruitment of DNA polymerases and other repair factors. Loss of RFC2 function or mutations can lead to genomic instability and resistance to DNA-damaging agents, making it a potential target for therapeutic intervention. Additionally, studies have suggested that RFC2 may have roles beyond DNA replication, including involvement in cellular responses to stress and regulation of transcription. Understanding the molecular mechanisms by which RFC2 operates and its interactions with other cellular components is vital for elucidating its function in both normal cellular physiology and pathological conditions. Consequently, ongoing research efforts continue to explore the multifaceted roles of RFC2, aiming to develop targeted strategies for cancer treatment and enhance our understanding of the underlying mechanisms of genome maintenance.
