Analytical Data
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Gene name
PTP1B
- Application
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Alternative Names
PTPN1;PTP1B;Tyrosine-Protein phosphatase non-receptor type 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P18031
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Expression Region
1-435aa
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AA Sequence
MEMEKEFEQIDKSGSWAAIYQDIRHEASDFPCRVAKLPKNKNRNRYRDVS PFDHSRIKLHQEDNDYINASLIKMEEAQRSYILTQGPLPNTCGHFWEMVW EQKSRGVVMLNRVMEKGSLKCAQYWPQKEEKEMIFEDTNLKLTLISEDIK SYYTVRQLELENLTTQETREILHFHYTTWPDFGVPESPASFLNFLFKVRE SGSLSPEHGPVVVHCSAGIGRSGTFCLADTCLLLMDKRKDPSSVDIKKVL LEMRKFRMGLIQTADQLRFSYLAVIEGAKFIMGDSSVQDQWKELSHEDLE PPPEHIPPPPRPPKRILEPHNGKCREFFPNHQWVKEETQEDKDCPIKEEK GSPLNAAPYGIESMSQDTEVRSRVVGGSLRGAQAASPAKGEPSLPEKDED HALSYWKPFLVNMCVATVLTAGAYLCYRFLFNSNT
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Molecular Weight
71 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PTP1B (Protein Tyrosine Phosphatase 1B) is a key enzyme involved in the regulation of various cellular signaling pathways, particularly those associated with insulin and leptin signaling. As a member of the protein tyrosine phosphatase family, PTP1B functions by dephosphorylating specific tyrosine residues on target proteins, which plays a crucial role in modulating cellular responses to growth factors and hormones. Its dysregulation has been implicated in various metabolic disorders, including obesity, insulin resistance, and type 2 diabetes. Consequently, PTP1B has garnered significant interest as a potential therapeutic target for the treatment of metabolic diseases. Researchers have been focusing on the expression and purification of recombinant PTP1B to study its structure, function, and interactions with other molecules in detail. Understanding the enzymatic mechanisms and regulatory pathways involving PTP1B can provide insights into its role in metabolic regulation and possibly lead to the development of novel treatment strategies that could improve insulin sensitivity and metabolic health. The production of recombinant PTP1B not only aids in elucidating its biochemical properties but also paves the way for high-throughput screening of small molecules that may inhibit its activity, thereby offering a promising approach for drug discovery in the fight against metabolic diseases.
