Analytical Data
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Gene name
MRPL4
- Application
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Alternative Names
Large ribosomal subunit protein uL4m. 39S ribosomal protein L4. mitochondrial. L4mt. MRP-L4
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BYD3
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Expression Region
1-263 aa
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AA Sequence
MLQFVRAGARAWLRPTGSQGLSSLAEEAARATENPEQVASEGLPEPVLRKVELPVPTHRRPVQAWVESLRGFEQERVGLADLHPDVFATAPRLDILHQVAMWQKNFKRISYAKTKTRAEVRGGGRKPWPQKGTGRARHGSIRSPLWRGGGVAHGPRGPTSYYYMLPMKVRALGLKVALTVKLAQDDLHIMDSLELPTGDPQYLTELAHYRRWGDSVLLVDLTHEEMPQSIVEATSRLKTFNLIPAVGEQRAQAPRVRMCRLRC
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Molecular Weight
54.67 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MRPL4, or Mitochondrial Ribosomal Protein L4, is a crucial component of the mitochondrial ribosome, playing a significant role in the protein synthesis machinery within mitochondria. Mitochondria are essential organelles responsible for energy production, and their function is tightly linked to cellular metabolism, growth, and apoptosis. Mutations or dysregulation of MRPL4 have been implicated in various mitochondrial diseases and metabolic disorders, highlighting its importance in human health. Recent studies have focused on characterizing the structural and functional properties of MRPL4 to understand its precise role in mitochondrial translation and its impact on mitochondrial function. The exploration of MRPL4 as a recombinant protein is of particular interest, as it offers potential insights into mitochondrial biogenesis and pathology. Advanced techniques, including cryo-electron microscopy and mass spectrometry, are being employed to elucidate the interactions between MRPL4 and mitochondrial RNA, as well as its interaction with other mitochondrial ribosomal proteins. Furthermore, understanding MRPL4's role in the context of mitochondrial dynamics and its involvement in oxidative stress responses can pave the way for developing therapeutic strategies for mitochondrial-related diseases. This research underscores the significance of MRPL4 not only as a fundamental molecular player in mitochondrial biology but also as a potential target for addressing mitochondrial dysfunctions that contribute to a range of human disorders.
