Analytical Data
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Gene name
SLC19A3
- Application
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Alternative Names
SLC19A3；Thiamine transporter 2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BZV2
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Expression Region
1-496aa
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AA Sequence
MDCYRTSLSSSWIYPTVILCLFGFFSMMRPSEPFLIPYLSGPDKNLTSAEITNEIFPVWTYSYLVLLLPVFVLTDYVRYKPVIILQGISFIITWLLLLFGQGVKTMQVVEFFYGMVTAAEVAYYAYIYSVVSPEHYQRVSGYCRSVTLAAYTAGSVLAQLLVSLANMSYFYLNVISLASVSVAFLFSLFLPMPKKSMFFHAKPSREIKKSSSVNPVLEETHEGEAPGCEEQKPTSEILSTSGKLNKGQLNSLKPSNVTVDVFVQWFQDLKECYSSKRLFYWSLWWAFATAGFNQVLNYVQILWDYKAPSQDSSIYNGAVEAIATFGGAVAAFAVGYVKVNWDLLGELALVVFSVVNAGSLFLMHYTANIWACYAGYLIFKSSYMLLITIAVFQIAVNLNVERYALVFGINTFIALVIQTIMTVIVVDQRGLNLPVSIQFLVYGSYFAVIAGIFLMRSMYITYSTKSQKDVQSPAPSENPDVSHPEEESNIIMSTKL
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Molecular Weight
55kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SLC19A3, a member of the solute carrier family, encodes a protein predominantly involved in the transport of various water-soluble vitamins, particularly thiamine (vitamin B1). Mutations in the SLC19A3 gene are associated with a rare neurological disorder known as biotin-responsive basal ganglia disease (BRBGD) and have been implicated in other neurological disorders, such as thiamine deficiency-related encephalopathy. The study of SLC19A3 recombinant protein aims to elucidate its transport mechanisms, substrate specificity, and the molecular basis of its associated pathologies. Understanding the structure-function relationship of SLC19A3 can provide insights into its role in cellular metabolism and potential therapeutic targets for conditions related to thiamine transport deficiency. Moreover, the production of recombinant SLC19A3 protein facilitates comprehensive biochemical assays and biophysical characterization, enabling researchers to explore the impact of various mutations on the protein’s function. This research not only enhances our understanding of thiamine transport in human health but also contributes to developing strategies for managing related diseases, making SLC19A3 a significant focus in biomedical research.
