Analytical Data
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基因名
PSAT1
- Application
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别名
PSAT1;PSA;Phosphoserine aminotransferase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q99K85
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表达区间
1-370aa
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氨基酸序列
MEATKQVVNFGPGPAKLPHSVLLEIQKQLLDYRGLGISVLEMSHRSSDFAKIIGNTENLVRELLAVPNNYKVIFVQGGGSGQFSAVPLNLIGLKAGRSADYVVTGAWSAKAAEEAKKFGTVNIVHPKLGSYTKIPDPSTWNLNPDASYVYFCANETVHGVEFDFVPDVKGAVLVCDMSSNFLSRPVDVSKFGVIFAGAQKNVGSAGVTVVIVRDDLLGFSLRECPSVLDYKVQAGNNSLYNTPPCFSIYVMGMVLEWIKNNGGAAAMEKLSSIKSQMIYEIIDNSQGFYVCPVERQNRSRMNIPFRIGNAKGDEALEKRFLDKAVELNMISLKGHRSVGGIRASLYNAVTTEDVEKLAAFMKNFLEMHQL
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分子量
56.5kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The PSAT1 (Phosphoserine Aminotransferase 1) is an enzyme that plays a critical role in the serine biosynthesis pathway, converting phosphoserine to serine, an essential amino acid involved in numerous biological processes, including protein synthesis, cell proliferation, and neurotransmitter production. Alterations in PSAT1 expression and activity have been linked to various diseases, particularly in cancer, where it is often upregulated to support the enhanced metabolic demands of rapidly dividing cells. The study of recombinant PSAT1 proteins is crucial for understanding the enzyme's structure-function relationships and its regulatory mechanisms. By producing these proteins in vitro, researchers can conduct detailed kinetic assays, explore interaction with other metabolic pathways, and investigate potential inhibitors that could serve as therapeutic agents. Furthermore, recombinant PSAT1 can be utilized to study its role in metabolic reprogramming in tumor cells, providing insights into cancer metabolism and potentially leading to novel treatment strategies. As the demand for serine in tumor biology increases, unraveling the detailed mechanisms of PSAT1 may reveal new targets for intervention, hence underscoring the importance of its research in the fields of biochemistry and molecular biology.
