Analytical Data
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Gene name
FAT1
- Application
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Alternative Names
FAT1；CDHF7；FAT；Protocadherin Fat 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q14517
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Expression Region
4203-4588aa
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AA Sequence
RKMISRKKKHQAEPKDKHLGPATAFLQRPYFDSKLNKNIYSDIPPQVPVRPISYTPSIPSDSRNNLDRNSFEGSAIPEHPEFSTFNPESVHGHRKAVAVCSVAPNLPPPPPSNSPSDSDSIQKPSWDFDYDTKVVDLDPCLSKKPLEEKPSQPYSARESLSEVQSLSSFQSESCDDNGYHWDTSDWMPSVPLPDIQEFPNYEVIDEQTPLYSADPNAIDTDYYPGGYDIESDFPPPPEDFPAADELPPLPPEFSNQFESIHPPRDMPAAGSLGSSSRNRQRFNLNQYLPNFYPLDMSEPQTKGTGENSTCREPHAPYPPGYQRHFEAPAVESMPMSVYASTASCSDVSACCEVESEVMMSDYESGDDGHFEEVTIPPLDSQQHTEV
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Molecular Weight
49.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
FAT1, a member of the cadherin superfamily, plays a vital role in various biological processes, including cell adhesion, migration, and differentiation. Research on FAT1 has gained momentum due to its implications in developmental biology and cancer. Aberrant expression of FAT1 has been linked to several malignancies, particularly in breast, colorectal, and other solid tumors, suggesting its potential as a tumor suppressor. The protein is involved in the regulation of signaling pathways that govern cellular behaviors, and its dysfunction may lead to unchecked cell proliferation and metastasis. As a result, FAT1 has emerged as a significant focus for novel therapeutic strategies and biomarker development. To further elucidate its functional mechanisms, researchers have undertaken the production and characterization of recombinant FAT1 proteins. These studies provide insights into the structural properties and regulatory functions of FAT1, enabling the exploration of its role in cellular context. Advances in techniques such as site-directed mutagenesis and protein crystallization allow for a deeper understanding of how FAT1 interacts with other cellular components. Ultimately, ongoing research on FAT1 and its recombinant forms aims to uncover the molecular underpinnings of its involvement in health and disease, presenting opportunities for targeted interventions in cancer treatment and regenerative medicine.
