Analytical Data
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基因名
PRNP
- Application
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别名
PRNP;ALTPRP;PRIP;PRP;Major prion Protein
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P04273
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表达区间
23-231aa
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氨基酸序列
KKRPKPGGWNTGGSRYPGQGSPGGNRYPPQGGGTWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQGGGTHNQWNKPSKPKTNMKHMAGAAAAGAVVGGLGGYMLGSAMSRPMMHFGNDWEDRYYRENMNRYPNQVYYRPVDQYNNQNNFVHDCVNITIKQHTVTTTTKGENFTETDIKIMERVVEQMCTTQYQKESQAYYDGRRS
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分子量
30.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of the prion protein gene (PRNP) recombinant protein has garnered significant attention in the field of molecular biology and neurodegenerative diseases, particularly in relation to prion diseases such as Creutzfeldt-Jakob disease and bovine spongiform encephalopathy. PRNP encodes the cellular prion protein (PrP), which exists in a primarily α-helical structure under normal conditions but can misfold into a pathogenic form, leading to neurodegeneration. Understanding the structural and functional characteristics of PRP is crucial for unraveling the mechanisms underlying prion propagation and pathogenesis. Recombinant PRNP proteins allow researchers to investigate the conformational changes, interactions with other cellular components, and the influence of mutations associated with prion diseases. These studies are essential for developing potential therapeutic strategies and diagnostic tools. Additionally, the production of recombinant PRNP enables the exploration of its role in normal physiology, immune response, and cellular signaling processes. Overall, ongoing research on PRNP recombinant protein represents a vital intersection of protein biochemistry, neurobiology, and therapeutic innovation, aiming to combat the devastating effects of prion-related disorders.
