Analytical Data
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Gene name
ACOX3
- Application
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Alternative Names
ACOX3；BRCOX；PRCOX；Peroxisomal acyl-coenzyme A oxidase 3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O15254
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Expression Region
1-624aa
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AA Sequence
MASTVEGGDTALLPEFPRGPLDAYRARASFSWKELALFTEGEGMLRFKKT IFSALENDPLFARSPGADLSLEKYRELNFLRCKRIFEYDFLSVEDMFKSP LKVPALIQCLGMYDSSLAAKYLLHSLVFGSAVYSSGSERHLTYIQKIFRM EIFGCFALTELSHGSNTKAIRTTAHYDPATEEFIIHSPDFEAAKFWVGNM GKTATHAVVFAKLCVPGDQCHGLHPFIVQIRDPKTLLPMPGVMVGDIGKK LGQNGLDNGFAMFHKVRVPRQSLLNRMGDVTPEGTYVSPFKDVRQRFGAS LGSLSSGRVSIVSLAILNLKLAVAIALRFSATRRQFGPTEEEEIPVLEYP MQQWRLLPYLAAVYALDHFSKSLFLDLVELQRGLASGDRSARQAELGREI HALASASKPLASWTTQQGIQECREACGGHGYLAMNRLGVLRDDNDPNCTY EGDNNILLQQTSNYLLGLLAHQVHDGACFRSPLKSVDFLDAYPGILDQKF EVSSVADCLDSAVALAAYKWLVCYLLRETYQKLNQEKRSGSSDFEARNKC QVSHGRPLALAFVELTVVQRFHEHVHQPSVPPSLRAVLGRLSALYALWSL SRHAALLYRAERRCSCPGRRDRSS
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Molecular Weight
77.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ACOX3 (Acyl-CoA oxidase 3) is a crucial enzyme located within the peroxisomes, primarily involved in the fatty acid beta-oxidation process. Research surrounding ACOX3 has gathered significance due to its association with various biochemical pathways and its potential impact on metabolic disorders. Studies have revealed that ACOX3 plays an essential role in the metabolism of medium- and long-chain fatty acids, which are vital for energy production and cellular function. Mutations or deficiencies in the ACOX3 gene can lead to disorders such as X-Linked Adrenoleukodystrophy, characterized by the accumulation of toxic fatty acids, resulting in neurodegeneration and adrenal insufficiency. Furthermore, the enzyme’s activity is linked to inflammatory responses and oxidative stress, which are implicated in various diseases, including obesity and cardiovascular conditions. As a result, understanding the structure and function of the recombinant ACOX3 protein has become a focal point in biochemistry and molecular biology, paving the way for potential therapeutic interventions. The production and characterization of ACOX3 as a recombinant protein facilitate in-depth studies of its enzymatic functions, regulatory mechanisms, and interactions with other cellular components. This research not only enhances our understanding of ACOX3's role in metabolic pathways but also opens avenues for developing enzyme replacement therapies and exploring its potential as a biomarker for metabolic disorders. The growing interest in peroxisomal biogenesis and lipid metabolism highlights the need for continued research on ACOX3, promising exciting developments in the fields of medical and translational research.
