Analytical Data
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基因名
ACOX3
- Application
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别名
ACOX3；BRCOX；PRCOX；Peroxisomal acyl-coenzyme A oxidase 3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O15254
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表达区间
1-624aa
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氨基酸序列
MASTVEGGDTALLPEFPRGPLDAYRARASFSWKELALFTEGEGMLRFKKT IFSALENDPLFARSPGADLSLEKYRELNFLRCKRIFEYDFLSVEDMFKSP LKVPALIQCLGMYDSSLAAKYLLHSLVFGSAVYSSGSERHLTYIQKIFRM EIFGCFALTELSHGSNTKAIRTTAHYDPATEEFIIHSPDFEAAKFWVGNM GKTATHAVVFAKLCVPGDQCHGLHPFIVQIRDPKTLLPMPGVMVGDIGKK LGQNGLDNGFAMFHKVRVPRQSLLNRMGDVTPEGTYVSPFKDVRQRFGAS LGSLSSGRVSIVSLAILNLKLAVAIALRFSATRRQFGPTEEEEIPVLEYP MQQWRLLPYLAAVYALDHFSKSLFLDLVELQRGLASGDRSARQAELGREI HALASASKPLASWTTQQGIQECREACGGHGYLAMNRLGVLRDDNDPNCTY EGDNNILLQQTSNYLLGLLAHQVHDGACFRSPLKSVDFLDAYPGILDQKF EVSSVADCLDSAVALAAYKWLVCYLLRETYQKLNQEKRSGSSDFEARNKC QVSHGRPLALAFVELTVVQRFHEHVHQPSVPPSLRAVLGRLSALYALWSL SRHAALLYRAERRCSCPGRRDRSS
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分子量
77.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ACOX3 (Acyl-CoA oxidase 3) is a crucial enzyme located within the peroxisomes, primarily involved in the fatty acid beta-oxidation process. Research surrounding ACOX3 has gathered significance due to its association with various biochemical pathways and its potential impact on metabolic disorders. Studies have revealed that ACOX3 plays an essential role in the metabolism of medium- and long-chain fatty acids, which are vital for energy production and cellular function. Mutations or deficiencies in the ACOX3 gene can lead to disorders such as X-Linked Adrenoleukodystrophy, characterized by the accumulation of toxic fatty acids, resulting in neurodegeneration and adrenal insufficiency. Furthermore, the enzyme’s activity is linked to inflammatory responses and oxidative stress, which are implicated in various diseases, including obesity and cardiovascular conditions. As a result, understanding the structure and function of the recombinant ACOX3 protein has become a focal point in biochemistry and molecular biology, paving the way for potential therapeutic interventions. The production and characterization of ACOX3 as a recombinant protein facilitate in-depth studies of its enzymatic functions, regulatory mechanisms, and interactions with other cellular components. This research not only enhances our understanding of ACOX3's role in metabolic pathways but also opens avenues for developing enzyme replacement therapies and exploring its potential as a biomarker for metabolic disorders. The growing interest in peroxisomal biogenesis and lipid metabolism highlights the need for continued research on ACOX3, promising exciting developments in the fields of medical and translational research.
