Analytical Data
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基因名
polA
- Application
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别名
polA；POLA；DNA polymerase alpha catalytic subunit
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P19821
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表达区间
293-832aa
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氨基酸序列
ALEEAPWPPPEGAFVGFVLSRKEPMWADLLALAAARGGRVHRAPEPYKALRDLKEARGLLAKDLSVLALREGLGLPPGDDPMLLAYLLDPSNTTPEGVARRYGGEWTEEAGERAALSERLFANLWGRLEGEERLLWLYREVERPLSAVLAHMEATGVRLDVAYLRALSLEVAEEIARLEAEVFRLAGHPFNLNSRDQLERVLFDELGLPAIGKTEKTGKRSTSAAVLEALREAHPIVEKILQYRELTKLKSTYIDPLPDLIHPRTGRLHTRFNQTATATGRLSSSDPNLQNIPVRTPLGQRIRRAFIAEEGWLLVALDYSQIELRVLAHLSGDENLIRVFQEGRDIHTETASWMFGVPREAVDPLMRRAAKTINFGVLYGMSAHRLSQELAIPYEEAQAFIERYFQSFPKVRAWIEKTLEEGRRRGYVETLFGRRRYVPDLEARVKSVREAAERKAFNMPVQGTAADLMKLAMVKLFPRLEEMGARMLLQVHDELVLEAPKERAEAVARLAKEVMEGVYPLAVPLEVEVGIGEDWLSAKE
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分子量
61.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PolA, also known as DNA polymerase I, plays a crucial role in DNA replication and repair processes in prokaryotic organisms, particularly in bacteria. As one of the primary enzymes involved in synthesizing DNA, PolA’s function extends beyond mere replication; it is vital for maintaining genomic stability and facilitating the repair of damaged DNA, particularly in response to environmental stresses. Research into PolA has garnered significant attention due to its potential implications in antibiotic resistance and bacterial pathogenesis. With the increasing prevalence of antibiotic-resistant strains, understanding the molecular mechanisms of PolA can provide insights into bacterial survival under adverse conditions, and may reveal new targets for antimicrobial drug development. Additionally, PolA's proofreading activity is essential for the fidelity of DNA replication, making it a key player in protecting organisms from mutations that could lead to malfunctions or diseases. Recent advances in structural biology have enabled researchers to elucidate the intricate mechanisms underlying PolA's enzymatic activity and its interactions with other proteins involved in DNA metabolism. This research not only enhances our understanding of basic molecular biology but also paves the way for biotechnological applications, such as the development of engineered DNA polymerases for use in synthetic biology and biotechnology. Overall, the study of PolA encompasses a broad spectrum of biological and medical relevance, highlighting its importance in both fundamental research and practical applications.
