Analytical Data
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基因名
MOGS
- Application
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别名
MOGS；GCS1；Mannosyl-oligosaccharide glucosidase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q13724
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表达区间
1-837aa
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氨基酸序列
MARGERRRRAVPAEGVRTAERAARGGPGRRDGRGGGPRSTAGGVALAVVVLSLALGMSGRWVLAWYRARRAVTLHSAPPVLPADSSSPAVAPDLFWGTYRPHVYFGMKTRSPKPLLTGLMWAQQGTTPGTPKLRHTCEQGDGVGPYGWEFHDGLSFGRQHIQDGALRLTTEFVKRPGGQHGGDWSWRVTVEPQDSGTSALPLVSLFFYVVTDGKEVLLPEVGAKGQLKFISGHTSELGDFRFTLLPPTSPGDTAPKYGSYNVFWTSNPGLPLLTEMVKSRLNSWFQHRPPGAPPERYLGLPGSLKWEDRGPSGQGQGQFLIQQVTLKIPISIEFVFESGSAQAGGNQALPRLAGSLLTQALESHAEGFRERFEKTFQLKEKGLSSGEQVLGQAALSGLLGGIGYFYGQGLVLPDIGVEGSEQKVDPALFPPVPLFTAVPSRSFFPRGFLWDEGFHQLVVQRWDPSLTREALGHWLGLLNADGWIGREQILGDEARARVPPEFLVQRAVHANPPTLLLPVAHMLEVGDPDDLAFLRKALPRLHAWFSWLHQSQAGPLPLSYRWRGRDPALPTLLNPKTLPSGLDDYPRASHPSVTERHLDLRCWVALGARVLTRLAEHLGEAEVAAELGPLAASLEAAESLDELHWAPELGVFADFGNHTKAVQLKPRPPQGLVRVVGRPQPQLQYVDALGYVSLFPLLLRLLDPTSSRLGPLLDILADSRHLWSPFGLRSLAASSSFYGQRNSEHDPPYWRGAVWLNVNYLALGALHHYGHLEGPHQARAAKLHGELRANVVGNVWRQYQATGFLWEQYSDRDGRGMGCRPFHGWTSLVLLAMAEDY
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分子量
91.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MOGS (Mannosyl-oligosaccharide glucosidase) is a critical enzyme involved in the glycoprocessing of nascent glycoproteins within the endoplasmic reticulum (ER). As an essential component of the quality control system in protein folding, MOGS specifically cleaves the glucose residues from oligosaccharides attached to glycoproteins, a crucial step before proteins are properly folded and transported to their functional destinations. Dysregulation or defects in MOGS have been linked to various diseases, including congenital disorders of glycosylation (CDG), which can lead to developmental issues and metabolic dysfunction. Research into MOGS and its recombinant forms is essential for understanding the underlying mechanisms of these diseases and developing potential therapeutic strategies. Recombinant MOGS proteins are being produced to elucidate the enzyme's structure-function relationship through biophysical and biochemical analyses. This research not only aims to clarify the role of MOGS in glycoprotein maturation but also explores its potential as a target for drug development, particularly for conditions arising from protein misfolding and glycosylation defects. Understanding the behavior of MOGS in various cellular contexts could pave the way for novel treatments and improve the management of related health disorders. The exploration of recombinant MOGS thus holds significant promise in both basic and applied biomedical research, contributing valuable insights into cellular mechanisms pertinent to glycoprotein synthesis and its implications in human health.
