Analytical Data
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Gene name
PPA2
- Application
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Alternative Names
PPA2;Inorganic pyrophosphatase 2. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9H2U2
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Expression Region
33-334aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSALYHTEE RGQPCSQNYR LFFKNVTGHY ISPFHDIPLK VNSKEENGIP MKKARNDEYE NLFNMIVEIP RWTNAKMEIA TKEPMNPIKQ YVKDGKLRYV ANIFPYKGYI WNYGTLPQTW EDPHEKDKST NCFGDNDPID VCEIGSKILS CGEVIHVKIL GILALIDEGE TDWKLIAINA NDPEASKFHD IDDVKKFKPG YLEATLNWFR LYKVPDGKPE NQFAFNGEFK NKAFALEVIK STHQCWKALL MKKCNGGAIN CTNVQISDSP FRCTQEEARS LVESVSSSPN KESNEEEQVW HFLGK
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Molecular Weight
37 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PPA2, or phosphoenolpyruvate carboxykinase 2, is an important enzyme involved in the metabolic pathway of gluconeogenesis, playing a critical role in glucose homeostasis. The study of PPA2 recombinant proteins has gained significant attention due to the enzyme's potential implications in various metabolic disorders, including diabetes and obesity. As a mitochondrial enzyme, PPA2 facilitates the conversion of oxaloacetate to phosphoenolpyruvate, which is essential for the synthesis of glucose from non-carbohydrate precursors. Understanding the function and regulation of PPA2 can contribute to the development of novel therapeutic strategies aimed at improving glucose management in patients with insulin resistance or impaired glucose tolerance. Additionally, the production of PPA2 recombinant proteins allows researchers to investigate the enzyme's structural properties and catalytic mechanisms, providing insights into its role in cellular metabolism. These studies can lead to enhanced comprehension of the metabolic pathways and their dysregulation in various pathophysiological conditions. By employing techniques such as gene cloning, expression in suitable host systems, and subsequent purification of the PPA2 recombinant protein, scientists can obtain a more detailed understanding of this enzyme's function and its potential as a drug target, ultimately paving the way for innovative approaches in metabolic disease treatment.
