Analytical Data
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Gene name
GTF3A
- Application
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Alternative Names
GTF3A；Transcription factor IIIA
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q92664
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Expression Region
1-365aa
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AA Sequence
MDPPAVVAESVSSLTIADAFIAAGESSAPTPPRPALPRRFICSFPDCSANYSKAWKLDAHLCKHTGERPFVCDYEGCGKAFIRDYHLSRHILTHTGEKPFVCAANGCDQKFNTKSNLKKHFERKHENQQKQYICSFEDCKKTFKKHQQLKIHQCQHTNEPLFKCTQEGCGKHFASPSKLKRHAKAHEGYVCQKGCSFVAKTWTELLKHVRETHKEEILCEVCRKTFKRKDYLKQHMKTHAPERDVCRCPREGCGRTYTTVFNLQSHILSFHEESRPFVCEHAGCGKTFAMKQSLTRHAVVHDPDKKKMKLKVKKSREKRSLASHLSGYIPPKRKQGQGLSLCQNGESPNCVEDKMLSTVAVLTLG
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Molecular Weight
41.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GTF3A, or General Transcription Factor 3A, is a critical component of the transcription machinery in eukaryotic cells, playing a significant role in the assembly of the transcription pre-initiation complex. It is essential for RNA polymerase II-mediated transcription, facilitating the recruitment of other transcription factors and RNA polymerase itself to the promoter regions of genes. The study of GTF3A and its recombinant protein has garnered attention due to its implications in fundamental biological processes and its potential link to various diseases, including cancer. The ability to produce recombinant GTF3A in vitro allows researchers to investigate its structural and functional properties, understand its interactions with other transcription factors, and evaluate its role in the regulation of gene expression. Additionally, the GTF3A protein could serve as a valuable tool in the development of gene therapies and novel strategies for cancer treatment, making it a focus of research in molecular biology and therapeutic applications. Understanding the dynamics of GTF3A, particularly through the use of recombinant technology, not only enhances our knowledge of transcription regulation but also opens up new avenues for drug discovery and the treatment of transcription-related disorders.
