Analytical Data
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Gene name
POLR2E
- Application
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Alternative Names
URI1;C19orf2;NNX3;PPP1R19;Unconventional prefoldin RPB5 interactor 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P19388
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Expression Region
1-210aa
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AA Sequence
MDDEEETYRL WKIRKTIMQL CHDRGYLVTQ DELDQTLEEF KAQSGDKPSE GRPRRTDLTV LVAHNDDPTD QMFVFFPEEP KVGIKTIKVY CQRMQEENIT RALIVVQQGM TPSAKQSLVD MAPKYILEQF LQQELLINIT EHELVPEHVV MTKEEVTELL ARYKLRENQL PRIQAGDPVA RYFGIKRGQV VKIIRPSETA GRYITYRLVQ
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
POLR2E, a subunit of RNA polymerase II, plays a crucial role in the transcription of protein-coding genes, which are essential for cell function and regulation. The study of POLR2E recombinant proteins has gained attention due to their implications in understanding the fundamental mechanisms of gene expression and their potential link to various diseases, including cancer. The POLR2E subunit is involved in the formation of the core enzyme responsible for synthesizing mRNA, and its proper function is vital for accurate transcription. Abnormalities or mutations in POLR2E can lead to defective transcription machinery, resulting in altered gene expression patterns that are associated with cellular dysfunction and oncogenesis. Researchers are focused on producing recombinant POLR2E proteins to investigate their structure, function, and interactions with other transcription factors and regulatory elements. This research not only enhances our understanding of the transcriptional process but also supports the development of targeted therapeutic strategies aimed at rectifying transcriptional dysregulation seen in various diseases. By elucidating the role of POLR2E in transcriptional regulation, scientists hope to discover novel insights into cellular biology and the underlying mechanisms of disease progression.
