Analytical Data
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Gene name
POLR2D
- Application
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Alternative Names
POLR2D;DNA-directed RNA polymerase II subunit RPB4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O15514
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Expression Region
1-142aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAAGGSD PRAGDVEEDA SQLIFPKEFE TAETLLNSEV HMLLEHRKQQ NESAEDEQEL SEVFMKTLNY TARFSRFKNR ETIASVRSLL LQKKLHKFEL ACLANLCPET AEESKALIPS LEGRFEDEEL QQILDDIQTK RSFQY
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Molecular Weight
19 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
POLR2D, a critical subunit of RNA polymerase II, plays a vital role in the transcription process, which is fundamental for gene expression regulation in eukaryotic cells. This enzyme is responsible for synthesizing messenger RNA (mRNA) from DNA templates, a crucial step in the flow of genetic information. The POLR2D subunit, specifically, is involved in the assembly of the polymerase complex and is essential for its stability and functionality. Research on POLR2D is significant because its dysregulation has been implicated in various diseases, including some cancers and neurodegenerative disorders. Understanding the structure and function of POLR2D through recombinant protein studies can provide insights into its specific roles in transcription regulation. By utilizing techniques such as protein expression, purification, and functional assays, scientists aim to elucidate the mechanistic pathways through which POLR2D and other subunits of RNA polymerase II interact and contribute to the transcriptional machinery. As a result, studying POLR2D not only contributes to our fundamental understanding of transcription but also opens avenues for potential therapeutic strategies targeting transcriptional dysregulation in disease contexts. The growing interest in post-translational modifications and the interaction of POLR2D with various transcription factors further enhances the importance of this research in elucidating the complexity of gene expression control. Overall, ongoing studies on recombinant POLR2D protein are anticipated to yield valuable information that could lead to advancements in molecular biology and medicine.
