Analytical Data
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Gene name
MCCC2
- Application
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Alternative Names
MCCC2；MCCB；Methylcrotonoyl-CoA carboxylase beta chain. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9HCC0
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Expression Region
23-563aa
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AA Sequence
YHGDSVAS LGTQPDLGSA LYQENYKQMK ALVNQLHERV EHIKLGGGEK ARALHISRGK LLPRERIDNL IDPGSPFLEL SQFAGYQLYD NEEVPGGGII TGIGRVSGVE CMIIANDATV KGGAYYPVTV KKQLRAQEIA MQNRLPCIYL VDSGGAYLPR QADVFPDRDH FGRTFYNQAI MSSKNIAQIA VVMGSCTAGG AYVPAMADEN IIVRKQGTIF LAGPPLVKAA TGEEVSAEDL GGADLHCRKS GVSDHWALDD HHALHLTRKV VRNLNYQKKL DVTIEPSEEP LFPADELYGI VGANLKRSFD VREVIARIVD GSRFTEFKAF YGDTLVTGFA RIFGYPVGIV GNNGVLFSES AKKGTHFVQL CCQRNIPLLF LQNITGFMVG REYEAEGIAK DGAKMVAAVA CAQVPKITLI IGGSYGAGNY GMCGRAYSPR FLYIWPNARI SVMGGEQAAN VLATITKDQR AREGKQFSSA DEAALKEPII KKFEEEGNPY YSSARVWDDG IIDPADTRLV LGLSFSAALN APIEKTDFGI FRM
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Molecular Weight
61.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MCCC2, or Methylcrotonyl-CoA Carboxylase 2, is an essential enzyme involved in the catabolism of certain amino acids and fatty acids, specifically in the degradation of leucine and isoleucine. Deficiencies in MCCC2 have been linked to metabolic disorders such as 3-methylcrotonyl-CoA carboxylase deficiency, which can result in symptoms ranging from mild metabolic disturbances to severe neurological impairments. The study of MCCC2 recombinant protein has gained prominence as researchers seek to understand its structural and functional properties at a molecular level. By producing MCCC2 in recombinant systems, scientists can investigate its enzymatic activity, regulation, and interaction with other metabolic pathways. Additionally, this research has potential implications for developing therapeutic strategies aimed at correcting the metabolic defects associated with MCCC2 deficiencies. Characterizing the recombinant protein helps elucidate the mechanisms underlying its action and provides insights into the enzymatic processes involved in human metabolism. The advancement of biotechnological approaches allows for a deeper understanding of MCCC2, paving the way for novel diagnostic and treatment options focused on improving the quality of life for individuals affected by related metabolic disorders.
