Analytical Data
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Gene name
MIPEP
- Application
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Alternative Names
EC 3.4.24.59 ; HMIP; MIP; Mipep; MIPEP_HUMAN; Mitochondrial intermediate peptidase; Mitochondrial intermedieate peptide; OTTHUMP00000018121; OTTHUMP00000042292
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q99797
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Expression Region
36-713 aa
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AA Sequence
VSTSW SPVGAAFNVK PQGSRLDLFG ERRGLFGVPE LSAPEGFHIA QEKALRKTEL LVDRACSTPP GPQTVLIFDE LSDSLCRVAD LADFVKIAHP EPAFREAAEE ACRSIGTMVE KLNTNVDLYQ SLQKLLADKK LVDSLDPETR RVAELFMFDF EISGIHLDKE KRKRAVDLNV KILDLSSTFL MGTNFPNKIE KHLLPEHIRR NFTSAGDHII IDGLHAESPD DLVREAAYKI FLYPNAGQLK CLEELLSSRD LLAKLVGYST FSHRALQGTI AKNPETVMQF LEKLSDKLSE RTLKDFEMIR GMKMKLNPQN SEVMPWDPPY YSGVIRAERY NIEPSLYCPF FSLGACMEGL NILLNRLLGI SLYAEQPAKG EVWSEDVRKL AVVHESEGLL GYIYCDFFQR ADKPHQDCHF TIRGGRLKED GDYQLPVVVL MLNLPRSSRS SPTLLTPSMM ENLFHEMGHA MHSMLGRTRY QHVTGTRCPT DFAEVPSILM EYFANDYRVV NQFARHYQTG QPLPKNMVSR LCESKKVCAA ADMQLQVFYA TLDQIYHGKH PLRNSTTDIL KETQEKFYGL PYVPNTAWQL RFSHLVGYGA RYYSYLMSRA VASMVWKECF LQDPFNRAAG ERYRREMLAH GGGREPMLMV EGMLQKCPSV DDFVSALVSD LDLDFETFLM DSE
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Molecular Weight
80.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MIPEP (Mitochondrial Intermediate Peptidase) is a crucial enzyme involved in the processing of mitochondrial proteins, playing a significant role in mitochondrial biogenesis and function. Its primary function is to cleave precursor proteins imported into mitochondria, facilitating the maturation of these proteins into their active forms. Research into MIPEP has gained traction due to its potential implications in various diseases, particularly those linked to mitochondrial dysfunctions, such as neurodegenerative disorders and metabolic syndromes. As mitochondria are the powerhouse of the cell, responsible for energy production and regulation of cellular metabolism, any aberration in MIPEP can lead to severe consequences for cellular health. Recent studies have also highlighted the enzyme's involvement in the mitochondrial stress response, suggesting that MIPEP may play a protective role under conditions of oxidative stress. Given the importance of mitochondrial health in overall cellular function, understanding the mechanisms and regulatory pathways involving MIPEP is critical for therapeutic interventions. Through the characterization of MIPEP and the development of recombinant proteins, researchers aim to explore its structural and functional properties, paving the way for potential applications in drug development and disease management. This research not only enhances our fundamental understanding of mitochondrial biology but also opens new avenues for targeting mitochondrial defects in human diseases, making MIPEP an attractive target for future studies in mitochondrial medicine.
