Analytical Data
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Gene name
PGM1
- Application
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Alternative Names
PGM1;Phosphoglucomutase-1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P36871
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Expression Region
1-562aa
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AA Sequence
MGSSHHHHHHSSGLVPRGSHMGSMVKIVTVKTQAYQDQKPGTSGLRKRVK VFQSSANYAENFIQSIISTVEPAQRQEATLVVGGDGRFYMKEAIQLIARI AAANGIGRLVIGQNGILSTPAVSCIIRKIKAIGGIILTASHNPGGPNGDF GIKFNISNGGPAPEAITDKIFQISKTIEEYAVCPDLKVDLGVLGKQQFDL ENKFKPFTVEIVDSVEAYATMLRSIFDFSALKELLSGPNRLKIRIDAMHG VVGPYVKKILCEELGAPANSAVNCVPLEDFGGHHPDPNLTYAADLVETMK SGEHDFGAAFDGDGDRNMILGKHGFFVNPSDSVAVIAANIFSIPYFQQTG VRGFARSMPTSGALDRVASATKIALYETPTGWKFFGNLMDASKLSLCGEE SFGTGSDHIREKDGLWAVLAWLSILATRKQSVEDILKDHWQKYGRNFFTR YDYEEVEAEGANKMMKDLEALMFDRSFVGKQFSANDKVYTVEKADNFEYS DPVDGSISRNQGLRLIFTDGSRIVFRLSGTGSAGATIRLYIDSYEKDVAK INQDPQVMLAPLISIALKVSQLQERTGRTAPTVIT
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Molecular Weight
64 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Phosphoglucomutase 1 (PGM1) is a key enzyme involved in carbohydrate metabolism, catalyzing the interconversion between glucose-1-phosphate and glucose-6-phosphate. Its significance extends beyond basic biochemical processes; alterations in PGM1 activity have been linked to various metabolic disorders, including glycogen storage diseases and potential implications in cancer metabolism. Research on PGM1 recombinant proteins has gained momentum due to their potential applications in understanding metabolic pathways and developing therapeutic strategies. Advances in recombinant DNA technology have enabled the production of PGM1 in various host systems, facilitating detailed studies on its structural and functional characteristics. Investigating the properties of the recombinant PGM1 protein provides insights into enzyme kinetics, substrate specificity, and interactions with inhibitors or activators. Moreover, understanding the regulatory mechanisms governing PGM1 activity could reveal novel targets for drug development. By elucidating the role of PGM1 in cellular metabolism, researchers aim to address the challenges associated with metabolic diseases and improve our understanding of energy homeostasis in different physiological and pathological contexts. Overall, the study of recombinant PGM1 protein represents a promising avenue for both basic research and translational applications in biomedicine.
