Analytical Data
-
Gene name
PGLS
- Application
-
Alternative Names
PGLS;6-phosphogluconolactonase
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
O95336
-
Expression Region
1-258aa
-
AA Sequence
MGSSHHHHHH SSGLVPRGSH MAAPAPGLIS VFSSSQELGA ALAQLVAQRA ACCLAGARAR FALGLSGGSL VSMLARELPA AVAPAGPASL ARWTLGFCDE RLVPFDHAES TYGLYRTHLL SRLPIPESQV ITINPELPVE EAAEDYAKKL RQAFQGDSIP VFDLLILGVG PDGHTCSLFP DHPLLQEREK IVAPISDSPK PPPQRVTLTL PVLNAARTVI FVATGEGKAA VLKRILEDQE ENPLPAALVQ PHTGKLCWFL DEAAARLLTV PFEKHSTL
-
Molecular Weight
30 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PGLS (phosphogluconate dehydrogenase-like protein) is an enzyme that plays a crucial role in the pentose phosphate pathway, a metabolic pathway important for cellular processes such as nucleic acid synthesis and redox regulation. Researchers have shown increasing interest in PGLS due to its potential implications in various biological processes, including cancer metabolism and oxidative stress responses. The study of recombinant PGLS proteins allows for a deeper understanding of their structure-function relationships, as well as their enzymatic mechanisms. By expressing PGLS in a suitable expression system, scientists can obtain large quantities of the protein for biochemical analysis and functional assays. This recombinant approach enables the investigation of PGLS’s substrate specificity, catalytic efficiency, and regulatory mechanisms, which are vital for understanding its role in metabolism and potential therapeutic applications. Furthermore, insights gained from these studies can pave the way for the development of novel drugs targeting metabolic disorders and cancer, where altered PGLS activity may play a significant role. Overall, the research surrounding recombinant PGLS is expanding, with the aim of elucidating the enzyme's functions and uncovering its significance in health and disease.
