Analytical Data
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Gene name
MEST
- Application
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Alternative Names
MEST; PEG1; Mesoderm-specific transcript homolog protein; Paternally-expressed gene 1 protein
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5EB52
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Expression Region
1-335 aa
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AA Sequence
MVRRDRLRRMREWWVQVGLLAVPLLAAYLHIPPPQLSPALHSWKSSGKFFTYKGLRIFYQDSVGVVGSPEIVVLLHGFPTSSYDWYKIWEGLTLRFHRVIALDFLGFGFSDKPRPHHYSIFEQASIVEALLRHLGLQNRRINLLSHDYGDIVAQELLYRYKQNRSGRLTIKSLCLSNGGIFPETHRPLLLQKLLKDGGVLSPILTRLMNFFVFSRGLTPVFGPYTRPSESELWDMWAGIRNNDGNLVIDSLLQYINQRKKFRRRWVGALASVTIPIHFIYGPLDPVNPYPEFLELYRKTLPRSTVSILDDHISHYPQLEDPMGFLNAYMGFINSF
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Molecular Weight
65.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MEST, or mesoderm-specific transcript, is a gene that encodes a protein implicated in various biological processes, including embryonic development and tumorigenesis. First identified in the context of mouse embryos, MEST exhibits imprinted expression, meaning it is expressed in a parent-of-origin-specific manner. This characteristic has drawn attention to its potential role in growth regulation and its association with certain diseases, particularly in the context of tumors and metabolic disorders. Recent research has focused on the reconstitution of the MEST protein to understand its functional mechanisms and interactions within signaling pathways. Given that MEST is linked to insulin resistance and obesity, its study is pivotal in the exploration of metabolic syndromes and related conditions. Furthermore, as cancer research evolves, understanding MEST's involvement in cellular growth and differentiation can provide insights into novel therapeutic targets. The recombinant expression and characterization of MEST protein has opened avenues for potential applications in biotechnology and medicine, emphasizing the importance of understanding both its physiological and pathological roles in human health.
