Analytical Data
-
基因名
PGAM1
- Application
-
别名
PGAM1;PGAMA;Phosphoglycerate mutase 1
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P18669
-
表达区间
1-254aa
-
氨基酸序列
MGSSHHHHHH SSGLVPRGSH MAAYKLVLIR HGESAWNLEN RFSGWYDADL SPAGHEEAKR GGQALRDAGY EFDICFTSVQ KRAIRTLWTV LDAIDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEA QVKIWRRSYD VPPPPMEPDH PFYSNISKDR RYADLTEDQL PSCESLKDTI ARALPFWNEE IVPQIKEGKR VLIAAHGNSL RGIVKHLEGL SEEAIMELNL PTGIPIVYEL DKNLKPIKPM QFLGDEETVR KAMEAVAAQG KAKK
-
分子量
31 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PGAM1 (Phosphoglycerate Mutase 1) is a crucial enzyme in the glycolytic pathway, catalyzing the conversion of 3-phosphoglycerate to 2-phosphoglycerate, which is essential for cellular energy production and metabolism. Abnormal expression of PGAM1 has been implicated in various pathological conditions, including cancer, where it has been identified as a potential biomarker and therapeutic target. The study of recombinant PGAM1 protein has gained significance due to its role in understanding the enzyme's biochemical properties, regulatory mechanisms, and its interaction with other metabolic pathways. Researchers are interested in producing recombinant PGAM1 to investigate its structure, function, and the effects of post-translational modifications on enzymatic activity. This knowledge can provide insights into metabolic dysregulation in diseases and advance the development of novel diagnostic and therapeutic strategies. Furthermore, recombinant PGAM1 can serve as a tool for screening small-molecule inhibitors that may have anti-cancer properties, highlighting its importance in both basic research and clinical applications. The growing interest in PGAM1 necessitates robust methods for its expression and purification, which can facilitate further studies and enhance our understanding of its biological significance.
