Analytical Data
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基因名
MEFV
- Application
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别名
FMF; Marenostrin; Mediterranean fever; Mediterranean fever protein; MEF; Mefv; MEFV_HUMAN; Pyrin; TRIM20
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O15553
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表达区间
375-781aa
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氨基酸序列
CKRHLKQVQLLFCEDHDEPICLICSLSQEHQGHRVRPIEEVALEHK KKIQKQLEHLKKLRKSGEEQRSYGEEKAVSFLKQTEALKQRVQRKLEQVYYFLEQQEHFF VASLEDVGQMVGQIRKAYDTRVSQDIALLDALIGELEAKECQSEWELLQDIGDILHRAKT VPVPEKWTTPQEIKQKIQLLHQKSEFVEKSTKYFSETLRSEMEMFNVPELIGAQAHAVNV ILDAETAYPNLIFSDDLKSVRLGNKWERLPDGPQRFDSCIIVLGSPSFLSGRRYWEVEVG DKTAWILGACKTSISRKGNMTLSPENGYWVVIMMKENEYQASSVPPTRLLIKEPPKRVGI FVDYRVGSISFYNVTARSHIYTFASCSFSGPLQPIFSPGTRDGGKNTAPLTICPVGGQGPD
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分子量
86,4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MEFV, the gene responsible for familial Mediterranean fever (FMF), encodes for the protein pyrin, which plays a crucial role in the regulation of inflammation and the innate immune response. Mutations in the MEFV gene are linked to FMF, a hereditary autoinflammatory disorder characterized by recurrent episodes of fever and serositis. Research into MEFV recombinant proteins aims to elucidate the functional mechanisms of pyrin, particularly how it interacts with other proteins in the inflammasome pathway, which is crucial for the activation of pro-inflammatory cytokines like IL-1β. Understanding the structure and function of the MEFV protein can provide insights into the pathophysiology of FMF and similar autoinflammatory conditions. Moreover, investigating MEFV-derived recombinant proteins holds potential for therapeutic applications, such as the development of targeted biologics that could alleviate the symptoms of FMF or other related disorders by modulating the inflammatory response. Recent advancements in molecular biology techniques have facilitated the production and characterization of recombinant MEFV proteins, enabling detailed studies of their biochemical properties and interactions. This line of research is pivotal for developing innovative treatment strategies and improving patient outcomes for individuals afflicted with FMF and related inflammatory diseases.
