Analytical Data
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Gene name
MDH1B
- Application
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Alternative Names
MDH1B; Putative malate dehydrogenase 1B; EC 1.1.1.-
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5I0G3
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Expression Region
1-518aa
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AA Sequence
MAKFVIAGRA DCPYYAKTEL VADYLQKNLP DFRIHKITQR PEVWEDWLKD VCEKNKWSHK NSPIIWRELL DRGGKGLLLG GYNEFLEHAQ LYYDVTSSMT TELMMVIAQE NLGAHIEKEQ EEEALKTCIN PLQVWITSAS APACYNLIPI LTSGEVFGMH TEISITLFDN KQAEEHLKSL VVETQDLASP VLRSVSICTK VEEAFRQAHV IVVLDDSTNK EVFTLEDCLR SRVPLCRLYG YLIEKNAHES VRVIVGGRTF VNLKTVLLMR YAPRIAHNII AVALGVEGEA KAILARKLKT APSYIKDVII WGNISGNNYV DLRKTRVYRY ESAIWGPLHY SRPVLNLIFD SEWVKREFVA ILKNLTTTGR QFGGILAAHS IATTLKYWYH GSPPGEIVSL GILSEGQFGI PKGIVFSMPV KFENGTWVVL TDLKDVEISE QIMTRMTSDL IQEKLVALGD KIHFQPYQSG HKDLVPDEEK NLAMSDAAEF PNQIPQTTFE KPQSLEFLNE FEGKTVES
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Molecular Weight
58,6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MDH1B, or Malate Dehydrogenase 1B, is a key enzyme involved in the citric acid cycle, playing a crucial role in cellular metabolism by catalyzing the reversible conversion of malate to oxaloacetate. Research on MDH1B is gaining momentum due to its potential implications in various physiological and pathological processes, including metabolic disorders, cancer development, and neurodegenerative diseases. Studies have shown that alterations in MDH1B activity can influence cellular respiration and energy production, which are critical in maintaining cellular homeostasis. Furthermore, the enzyme is of interest in the context of plant biology, as it contributes to stress responses and adaptation mechanisms. Recent advances in biotechnology have facilitated the recombinant expression of MDH1B, allowing researchers to obtain large quantities of the protein for in-depth studies. Understanding the structure and function of MDH1B through recombinant protein techniques not only enhances our comprehension of its role in metabolism but also aids in the identification of potential therapeutic targets. The exploration of MDH1B's physiological functions and its involvement in disease mechanisms presents promising avenues for the development of novel treatments, further underscoring the importance of this enzyme in both health and disease.
