Analytical Data
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Gene name
MDFI
- Application
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Alternative Names
MDFI; MyoD family inhibitor; Myogenic repressor I-mf
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q99750
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Expression Region
1-246aa
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AA Sequence
MYQVSGQRPS GCDAPYGAPS AAPGPAQTLS LLPGLEVVTG STHPAEAAPE EGSLEEAATP MPQGNGPGIP QGLDSTDLDV PTEAVTCQPQ GNPLGCTPLL PNDSGHPSEL GGTRRAGNGA LGGPKAHRKL QTHPSLASQG SKKSKSSSKS TTSQIPLQAQ EDCCVHCILS CLFCEFLTLC NIVLDCATCG SCSSEDSCLC CCCCGSGECA DCDLPCDLDC GILDACCESA DCLEICMECC GLCFSS
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Molecular Weight
25 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MDFI, or Myeloid Differentiation Factor 1, is a critical protein involved in various cellular processes, including myeloid differentiation, immune response modulation, and apoptosis. Research into MDFI has gained momentum due to its potential implications in cancer biology and autoimmune diseases. Notably, its expression patterns and functions suggest a role in the development and progression of certain malignancies, as well as in the regulation of inflammatory responses. Scientists have been particularly interested in understanding how MDFI interacts with other cellular pathways and proteins, which could reveal novel therapeutic targets. Recent studies have demonstrated that modulating MDFI levels can influence cellular functions, making it a promising candidate for drug development in treating hematological malignancies and autoimmune disorders. Moreover, understanding the structural biology of MDFI through techniques such as X-ray crystallography and cryo-electron microscopy could provide insights into its functional mechanisms. As such, ongoing research aims to elucidate the precise biological roles of MDFI, its molecular interactions, and its potential as a biomarker, thereby advancing our knowledge in both basic and clinical sciences related to immunology and oncology.
