Analytical Data
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Gene name
PDZK1
- Application
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Alternative Names
PDZK1;CAP70;NHERF3;PDZD1;Na(+)/H(+) exchange regulatory cofactor NHE-RF3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5T2W1
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Expression Region
1-519aa
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AA Sequence
MTSTFNPRECKLSKQEGQNYGFFLRIEKDTEGHLVRVVEKCSPAEKAGLQDGDRVLRINGVFVDKEEHMQVVDLVRKSGNSVTLLVLDGDSYEKAVKTRVDLKELGQSQKEQGLSDNILSPVMNGGVQTWTQPRLCYLVKEGGSYGFSLKTVQGKKGVYMTDITPQGVAMRAGVLADDHLIEVNGENVEDASHEEVVEKVKKSGSRVMFLLVDKETDKRHVEQKIQFKRETASLKLLPHQPRIVEMKKGSNGYGFYLRAGSEQKGQIIKDIDSGSPAEEAGLKNNDLVVAVNGESVETLDHDSVVEMIRKGGDQTSLLVVDKETDNMYRLAHFSPFLYYQSQELPNGSVKEAPAPTPTSLEVSSPPDTTEEVDHKPKLCRLAKGENGYGFHLNAIRGLPGSFIKEVQKGGPADLAGLEDEDVIIEVNGVNVLDEPYEKVVDRIQSSGKNVTLLVCGKKAYDYFQAKKIPIVSSLADPLDTPPDSKEGIVVESNHDSHMAKERAHSTASHSSSNSEDTEM
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Molecular Weight
61.1kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PDZK1 (PDZ domain containing 1) is a key scaffolding protein involved in various cellular processes, including signal transduction, cell adhesion, and the maintenance of cell polarity. It is composed of multiple PDZ domains that facilitate protein-protein interactions, allowing it to assemble signaling complexes and regulate the localization of its partner proteins. Research into PDZK1 has gained prominence due to its crucial role in the functions of epithelial tissues and its implications in various diseases, particularly in cancer and kidney disorders. Dysregulation of PDZK1 has been associated with altered cell signaling pathways, contributing to tumor progression and metastasis. The expression of PDZK1 is also linked to the function of epithelial sodium channels (ENaC), highlighting its relevance in renal physiology. Recent studies have focused on the structural characterization of PDZK1, aiming to understand the molecular mechanisms underpinning its interactions and biological functions. Advances in recombinant protein technology have enabled the production of PDZK1 for biochemical and structural analyses, facilitating the exploration of its role in disease and potential therapeutic targeting. Understanding the function of PDZK1 at a molecular level may provide insights into novel strategies for treating conditions associated with its dysregulation.
