Analytical Data
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Gene name
ERCC3
- Application
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Alternative Names
ERCC3；XPB；XPBC；General transcription and DNA repair factor IIH helicase/translocase subunit XPB
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P19447
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Expression Region
1-782aa
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AA Sequence
MGKRDRADRDKKKSRKRHYEDEEDDEEDAPGNDPQEAVPSAAGKQVDESGTKVDEYGAKDYRLQMPLKDDHTSRPLWVAPDGHIFLEAFSPVYKYAQDFLVAIAEPVCRPTHVHEYKLTAYSLYAAVSVGLQTSDITEYLRKLSKTGVPDGIMQFIKLCTVSYGKVKLVLKHNRYFVESCHPDVIQHLLQDPVIRECRLRNSEGEATELITETFTSKSAISKTAESSGGPSTSRVTDPQGKSDIPMDLFDFYEQMDKDEEEEEETQTVSFEVKQEMIEELQKRCIHLEYPLLAEYDFRNDSVNPDINIDLKPTAVLRPYQEKSLRKMFGNGRARSGVIVLPCGAGKSLVGVTAACTVRKRCLVLGNSAVSVEQWKAQFKMWSTIDDSQICRFTSDAKDKPIGCSVAISTYSMLGHTTKRSWEAERVMEWLKTQEWGLMILDEVHTIPAKMFRRVLTIVQAHCKLGLTATLVREDDKIVDLNFLIGPKLYEANWMELQNNGYIAKVQCAEVWCPMSPEFYREYVAIKTKKRILLYTMNPNKFRACQFLIKFHERRNDKIIVFADNVFALKEYAIRLNKPYIYGPTSQGERMQILQNFKHNPKINTIFISKVGDTSFDLPEANVLIQISSHGGSRRQEAQRLGRVLRAKKGMVAEEYNAFFYSLVSQDTQEMAYSTKRQRFLVDQGYSFKVITKLAGMEEEDLAFSTKEEQQQLLQKVLAATDLDAEEEVVAGEFGSRSSQASRRFGTMSSMSGADDTVYMEYHSSRSKAPSKHVHPLFKRFRK
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Molecular Weight
115.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ERCC3, also known as XPB, is a crucial gene involved in the nucleotide excision repair (NER) pathway, which is essential for the repair of DNA damage caused by ultraviolet (UV) light and other environmental factors. Mutations in the ERCC3 gene are associated with Xeroderma Pigmentosum (XP), a condition characterized by extreme sensitivity to sunlight, leading to a significantly increased risk of skin cancer. Given the role of ERCC3 in maintaining genomic stability, researchers have focused on its recombinant protein to better understand its structure and function in DNA repair mechanisms. The study of ERCC3 recombinant protein provides insights into the molecular basis of NER, facilitating the development of therapeutic strategies for conditions related to deficient DNA repair. Additionally, understanding the biochemical properties of ERCC3 can aid in elucidating the interplay between various components of the NER machinery, potentially revealing novel targets for cancer treatment and prevention. The investigation of ERCC3 also extends to its role in other cellular processes, such as transcription regulation and responses to oxidative stress, making it a significant focus in cancer biology and genetic research. As scientists continue to explore the functional complexities of ERCC3 and its interactions with other proteins, the potential for advancing clinical applications in genetic disorders and tumor therapies remains a promising frontier in molecular biology.
