Analytical Data
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基因名
EIF4A1
- Application
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别名
EIF4A1；DDX2A；EIF4A；Eukaryotic initiation factor 4A-I
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P60842
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表达区间
2-406aa
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氨基酸序列
SASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPSDVLEVTKKFMRDPIRILVKKEELTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVSAMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMPLNVADLI
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分子量
53.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EIF4A1, or Eukaryotic Translation Initiation Factor 4A1, is a vital protein involved in the initiation phase of eukaryotic protein synthesis. It functions as an RNA helicase, unwinding mRNA secondary structures to facilitate ribosome binding and translation. Given its critical role in gene expression, EIF4A1 is implicated in various cellular processes, including proliferation, differentiation, and stress responses. Alterations in EIF4A1 activity have been associated with several diseases, particularly cancer, where its overexpression is often linked to enhanced tumor growth and metastasis. As a result, EIF4A1 has emerged as a potential therapeutic target in cancer treatment, making its recombinant protein a valuable tool for studying its function and regulation. Researchers are utilizing recombinant EIF4A1 to explore its interactions with other translation factors, RNA, and small molecule inhibitors. This research aims to elucidate the mechanistic pathways governing protein synthesis and highlight the implications of EIF4A1 in disease. The ability to produce and study recombinant EIF4A1 in vitro can significantly advance our understanding of translational control and open new avenues for therapeutic interventions in conditions where EIF4A1 is dysregulated.
