Analytical Data
-
Gene name
sucB
- Application
-
Alternative Names
sucB；Succinate--CoA ligase [GDP-forming] subunit beta. mitochondrial
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P0AFG7
-
Expression Region
2-405aa
-
AA Sequence
SSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQQASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAPAAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPTRLLLDV
-
Molecular Weight
49.9 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SucB, or succinyl-CoA synthetase beta subunit, is a crucial enzyme involved in the citric acid cycle, playing a significant role in energy metabolism by facilitating the conversion of succinyl-CoA to succinate while generating GTP. This enzyme is of particular interest in microbial and plant systems, where it contributes to anaerobic respiration and fermentation processes. Studies have shown that SucB is not only pivotal for metabolic flux but also influences the overall growth and adaptation of organisms to varying environmental conditions. The recombinant expression of SucB allows for detailed characterization of its enzymatic properties, including kinetics and substrate specificity. Understanding SucB through recombinant protein studies can provide insights into its functional mechanisms and regulatory roles within metabolic pathways. Moreover, manipulating SucB’s activity or expression could have biotechnological applications, particularly in biofuel production and metabolic engineering strategies aimed at enhancing yield and efficiency in microbial systems. Therefore, research on SucB recombinant protein is crucial for elucidating its biological significance and exploiting its potential in industrial applications. The advancements in protein expression technologies and structural biology tools have further enabled the comprehensive analysis of SucB, paving the way for innovative approaches to optimize metabolic processes in various biological systems.
