Analytical Data
-
基因名
P4HB
- Application
-
别名
P4HB;ERBA2L;PDI;PDIA1;Protein disulfide-isomerase
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P07237
-
表达区间
18-508aa
-
氨基酸序列
MGSSHHHHHH SSGLVPRGSH MDAPEEEDHV LVLRKSNFAE ALAAHKYLLV EFYAPWCGHC KALAPEYAKA AGKLKAEGSE IRLAKVDATE ESDLAQQYGV RGYPTIKFFR NGDTASPKEY TAGREADDIV NWLKKRTGPA ATTLPDGAAA ESLVESSEVA VIGFFKDVES DSAKQFLQAA EAIDDIPFGI TSNSDVFSKY QLDKDGVVLF KKFDEGRNNF EGEVTKENLL DFIKHNQLPL VIEFTEQTAP KIFGGEIKTH ILLFLPKSVS DYDGKLSNFK TAAESFKGKI LFIFIDSDHT DNQRILEFFG LKKEECPAVR LITLEEEMTK YKPESEELTA ERITEFCHRF LEGKIKPHLM SQELPEDWDK QPVKVLVGKN FEDVAFDEKK NVFVEFYAPW CGHCKQLAPI WDKLGETYKD HENIVIAKMD STANEVEAVK VHSFPTLKFF PASADRTVID YNGERTLDGF KKFLESGGQD GAGDDDDLED LEEAEEPDME EDDDQKAVKD EL
-
分子量
57 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
P4HB, or prolyl 4-hydroxylase beta subunit, is a critical enzyme involved in collagen synthesis and post-translational modification. Research on P4HB has gained significance due to its essential role in various physiological processes, including wound healing, fibrosis, and tissue repair. The enzyme's activity is closely linked to the stabilization of collagen triple helices, making it a pivotal factor in ensuring the structural integrity of extracellular matrix components. Dysregulation of P4HB has been implicated in several pathological conditions, such as fibrotic diseases and cancer, where excessive collagen deposition occurs. Understanding the functional mechanisms and regulation of P4HB could provide therapeutic insights into these conditions. Furthermore, as a target for drug development, modulating its activity may offer new treatment strategies for diseases characterized by altered collagen metabolism. Given the increasing interest in tissue engineering and regenerative medicine, studies on P4HB are also expanding, aiming to harness its potential to improve biomaterials and promote healing. Overall, the exploration of P4HB as a research focus highlights its multifaceted roles in health and disease, paving the way for novel therapeutic interventions.
